Isolation and characterization of Df(1)BSC530
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC530 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03741 and P{XP}CG13369d06819. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f03741/P{XP}CG13369d06819; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC530 from the segment of PBac{WH}f03741 to the left of its FRT site and the segment of P{XP}CG13369d06819 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC530 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 1A5;1B12. It failed to complement ac4.