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Cook, K., Christensen, S., Cook, K. (2008.7.11). Isolation and characterization of Df(3R)BSC569. 
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FBrf0205015
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From: 	Kevin Cook <kcook@XXXX>
Subject: 	Isolation and characterization of Df(3R)BSC569
Date: 	Fri, 11 Jul 2008  13:23:03  -0400  ( 18:23  BST)
Isolation and characterization of Df(3R)BSC569
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC569 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d03302 and PBac{RB}CG14876[e02690]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}d03302/PBac{RB}CG14876[e02690] males. The males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.RB3}BSC569 from the segment 
of P{XP}d03302 to the left of its FRT site and the segment of 
PBac{RB}CG14876[e02690] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. with the substitution of the primer 
5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer 
in the Hybrid PCR protocol in the Supplementary Methods. The 
cytological breakpoints of Df(3R)BSC569 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
89A1;89A5. Df(3R)BSC569 failed to complement Df(3R)ED5705 and Df(3R)Exel8162.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (3)
    Insertions (3)
    Transgenic Constructs (1)