Isolation and characterization of Df(3R)BSC611
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC611 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG9312f04951 and P{XP}rdxd06943. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C,Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG9312f04951/P{XP}rdxd06943 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC611 from the segment of PBac{WH}CG9312f04951 to the left of its FRT site and the segment of P{XP}rdxd06943 to the right of its FRT site. The cytological breakpoints of Df(3R)BSC611 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 87F13;88A4. Df(3R)BSC611 failed to complement Nsf2A6 and Df(3R)Exel6288.