Subject: personal communication to FlyBase:  origin of miscellaneous cell lines
Origin of miscellaneous cell lines.
Lucy Cherbas
Drosophila Genomics Resource Center, Indiana University
The cell lines G1 (FBtc0000016) and G2 (FBtc0000004) are embryonic lines made by Walter Gehring (with his own hands). He told me this when he gave me some G2 cells in the mid-1980s.
The cell line D1 (FBtc0000010) is an embryonic line made by Andreas Dubendorfer when he was a student in Jimmy Sang's lab in the mid-1970s; this information was conveyed by Dubendorfer to Peter Cherbas, along with a sample of the cells.
The lines CCa (FBtc0000175) and DX (FBtc0000176) are embryonic lines made by Vladimir Gvozdev somewhere around 1970. The cells were sent to me by Buzz Baum, but the information about their origin was confirmed directly by Gvozdev.
The line Sg4 (FBtc0000179) is a clone made from Schneider's line 2 (FBtc0000999) by Donna Arndt-Jovin in the early 90s by flow sorting for 2N DNA content; her Schneider's line 2 was obtained from Doberman. She called the line Sf4, but apparently a typo caused the name to be converted to Sg4 by the time it was first used in a publication, by Vincenzo Pirrotta's lab. She says that the starting Schneider's line 2 cells that she used were largely tetraploid and female, while the Sg4 cells were diploid and male. I obtained the cells from Pirrotta's lab, but all of the information reported here came directly from Arndt-Jovin.
Kc7E10 (FBtc0000182) is a clone made from Kc (0000998) by Dennis O'Connor around 1980. It was found by John and Liselot Fessler to make an unusually large amount of extracellular matrix and was used by the Fesslers and their collaborators as a source of extracellular matrix proteins for biochemical work. I obtained the cells and all of the information reported here from the Fesslers.
Lucy
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Lucy Cherbas
Department of Biology and
Drosophila Genomics Resource Center
Jordan Hall
1001 East Third Street
Indiana University
Bloomington, IN 47405