Isolation and characterization of Df(1)BSC647
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC647 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03971 and P{XP}CG32552d09154. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f03971/P{XP}CG32552d09154; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC647 from the segment of PBac{WH}f03971 to the left of its FRT site and the segment of P{XP}CG32552d09154 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f03971 to be Release 3 genomic coordinate 17442428 on the X chromosome, which corresponds to 16C3 on the Release 3 genome map and 16C1 on the Release 5 genome map.  Exelixis, Inc. determined the insertion site of P{XP}CG32552d09154 to be Release 3 genomic coordinate 17794134 on the X chromosome, which corresponds to 16F6 on both the Release 3 and Release 5 genome maps. Consequently, the cytological breakpoints of Df(1)BSC647 are predicted to be 16C1;16F6. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.