Isolation and characterization of Df(2R)BSC782
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC782 was isolated as a FLP recombinase-induced recombination event involving P{XP}betaTub56Dd07340 and PBac{WH}f04601. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}betaTub56Dd07340/PBac{WH}f04601 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC782 from the segment of P{XP}betaTub56Dd07340 to the left of its FRT site and the segment of PBac{WH}f04601 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2R)BSC782 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:15338532 ;15389309 and the cytological breakpoints predicted from these coordinates are 56D8;56D14. Df(2R)BSC782 failed to complement Df(2R)ED3728 and Df(2R)BSC22.