Isolation and characterization of Df(3L)BSC799
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC799 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}bab1f02681 and P{XP}d07664. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}bab1f02681/P{XP}d07664 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC799 from the segment of PBac{WH}bab1f02681 to the left of its FRT site and the segment of P{XP}d07664 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC799 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:1074839 ;1247708 and the cytological breakpoints predicted from these coordinates are 61E3;61F4. Df(3L)BSC799 failed to complement Klp61F07012.