Isolation and characterization of Df(2L)BSC811
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC811 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG3036d04335 and PBac{WH}f05123. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG3036d04335/PBac{WH}f05123 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC811 from the segment of P{XP}CG3036d04335 to the left of its FRT site and the segment of PBac{WH}f05123 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2L)BSC811 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:4892286 ;4955471 and the cytological breakpoints predicted from these coordinates are 25B1;25B4. Df(2L)BSC811 failed to complement betaggt-IS-2554 and Df(2L)Exel7021.