Isolation and characterization of Df(2L)BSC826
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC826 was isolated as a FLP recombinase-induced recombination event involving P{XP}l(2)01810d06403 and PBac{WH}Rab6f05135. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}l(2)01810d06403/PBac{WH}Rab6f05135 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC826 from the segment of P{XP}l(2)01810<up> d06403</up> to the left of its FRT site and the segment of PBac{WH}Rab6f05135 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2L)BSC826 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:12027460 ;12108831 and the cytological breakpoints predicted from these coordinates are 33B9;33C4. Df(2L)BSC826 failed to complement prd8 and Df(2L)ED775.