Isolation and characterization of Df(3L)BSC833
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC833 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05666 and PBac{WH}CG7304f05948. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d05666/PBac{WH}CG7304f05948 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC833 from the segment of P{XP}d05666 to the left of its FRT site and the segment of PBac{WH}CG7304f05948 to the right of its FRT site. The breakpoints of Df(3L)BSC833 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:15507245--15507523 ;15671057 and the cytological breakpoints predicted from these coordinates are 71D4;71F1. Df(3L)BSC833 failed to complement Df(3L)XG5 and Df(3L)ED218.