Isolation and characterization of Df(3L)BSC837
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC837 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07338 and PBac{WH}FucTAf03774. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d07338/PBac{WH}FucTAf03774 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC837 from the segment of P{XP}d07338 to the left of its FRT site and the segment of PBac{WH}FucTAf03774 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d07338 to be Release 3 genomic coordinate 14920996 on chromosome arm 3L. This corresponds to  3L:14965217  and 71A4 on the Release 5 map. The insertion site of PBac{WH}FucTAf03774 is Release 5 genomic coordinate  3L:15144157 , which corresponds to 71B6. Consequently, the molecular breakpoints of Df(3L)BSC837 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:14965217 ;15144157 and the cytological breakpoints predicted from these coordinates are 71A4;71B6. Df(3L)BSC837 failed to complement mndBG01434.