Isolation and characterization of Df(2R)BSC865
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC865 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG13527f04582 and P{XP}d10090. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG13527f04582/P{XP}d10090 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC865 from the segment of PBac{WH}CG13527f04582 to the left of its FRT site and the segment of P{XP}d10090 to the right of its FRT site. The breakpoints of Df(2R)BSC865 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:18652440 ;18828551 and the cytological breakpoints predicted from these coordinates are 59A4;59B7. Df(2R)BSC865 failed to complement Nup21410444 and Df(2R)BSC599. Df(2R)BSC865 heterozygotes have a Minute phenotype from deletion of RpL23.