FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UASp-Opto-LD}
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General Information
Symbol
P{UASp-Opto-LD}
FlyBase ID
FBtp0174321
Feature type
transgenic_transposable_element
Component Allele(s)
Atha\CIB1CIBN(ΔNLS).Opto-LD.UASp
Lsd-1Opto-LD.UASp
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Lsd-1Opto-LD.UASp
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Lsd-1
Tagged with
CRY2clust
mCherry
Description

UASp sequences regulate expression of a dicistronic transcript that encodes two open reading frames (ORFs) separated by a 2A linker (this results in separate translation of each protein). The first ORF encodes Lsd-1 that is tagged with mCherry and CRY2clust; the Lsd-1 sequence targets this protein to the lipid droplet membrane, with the mCherry and CRY2clust tags located in the cytoplasm. The second ORF encodes a motor domain (Tag:MT(rKif5a)) tagged with CIBN(ΔNLS). Together the two encoded proteins form an optogenetic tool for controlling the localization of lipid droplets: upon illumination with blue light the CIBN(ΔNLS) and CRY2clust motifs in the two proteins heterodimerize, recruiting the Tag:MT(rKif5a) motor to the lipid droplets and driving their transport towards microtubule plus ends.

(Deng et al., 2025)
Component allele Atha\CIB1CIBN(ΔNLS).Opto-LD.UASp
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
CIBN(ΔNLS)
Tagged with
Tag:MT(rKif5a)
Description

UASp sequences regulate expression of a dicistronic transcript that encodes two open reading frames (ORFs) separated by a 2A linker (this results in separate translation of each protein). The first ORF encodes Lsd-1 that is tagged with mCherry and CRY2clust; the Lsd-1 sequence targets this protein to the lipid droplet membrane, with the mCherry and CRY2clust tags located in the cytoplasm. The second ORF encodes a motor domain (Tag:MT(rKif5a)) fused to CIBN(ΔNLS). Together the two encoded proteins form an optogenetic tool for controlling the localization of lipid droplets: upon illumination with blue light the CIBN(ΔNLS) and CRY2clust motifs in the two proteins heterodimerize, recruiting the Tag:MT(rKif5a) motor to the lipid droplets and driving their transport towards microtubule plus ends.

(Deng et al., 2025)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-Opto-LD}
(FlyBase, 1992-)
UASp-Lsd-1-mCherry-CRY2P2A-KIF5A-CIBN
(Deng et al., 2025)
Secondary FlyBase IDs
    References (3)