FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: P{ncd.TSmod}
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General Information
Symbol
P{ncd.TSmod}
FlyBase ID
FBtp0175096
Feature type
transgenic_transposable_element
Component Allele(s)
ncdTSmod
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele ncdTSmod
Transgenic product class
wild_type
(Do et al., 2026)
Component
Use(s)
Regulatory region(s)
ncd
Encoded product / tool
ncd
Description

The ncd promoter drives expression of a fluorescence resonance energy transfer (FRET)-based sensor that is designed to measure tension along the kinesin motor encoded by the ncd gene. The sensor consists of the full-length, wild-type ncd coding sequence with a tension-sensing module (TSmod) inserted at K210 near the tail-stalk junction. The tension-sensing module comprises two fluorescent proteins - mTFP1 (FRET donor) and Venus (FRET acceptor) - separated from each other by a stretchable domain from spider silk (PMID:20613844). Under low tension high FRET is produced, but under high tension, the two fluorophores move further apart, reducing the FRET signal.

(Do et al., 2026)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
607194
w1118, P{ncd.TSmod}F38M1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{ncd.TSmod}
(FlyBase, 1992-)
TsNcd
(Do et al., 2026)
Secondary FlyBase IDs
    References (3)