FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

classical fluorescence spectroscopy

ID (Ontology)

MI:0017 (Molecular Interactions)

Definition

Proteins contain endogenous fluorophores such as tryptophan residue and heme or flavins groups. Protein folding and protein-protein interaction can be studied by monitoring changes in the tryptophan environment detected by changes in its intrinsic fluorescence. Changes in the fluorescence emission spectrum on complex formation can occur either due to a shift in the wavelength of maximum fluorescence emission or by a shift in fluorescence intensity caused by the mixing of two proteins. The interaction of two proteins causes a shift in the fluorescence emission spectrum relative to the sum of the individual fluorescence spectra, resulting in a difference spectrum [F (complex)-2 F (sum)], which is a measurable effect of the interaction. Loss of fluorescence signal from a substrate can be used to measure protein cleavage.

Also Known As

"fluorescence spectr"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
classical fluorescence spectroscopy (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  biophysical
   |__fluorescence technology
       |__classical fluorescence spectroscopy  12 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	fluorescence technology
Part of
Synonyms & Secondary IDs
Synonyms
	"fluorescence spectr" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts