FB2026_03 , released September 17, 2026
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Term protein cross-linking with a bifunctional reagent ID (Ontology) MI:0031 (Molecular Interactions)
Definition Cross-linking agents induce the formation of covalent bonds among proteins that are neighbours. The cross-linker may be a bifunctional molecule having two reactive ends linked by a spacer, often containing a disulfide bond. When a reducing agent is added the disulfide bridge is cleaved, the cross-linked pairs are released and can be identified. There are various classes of cross-linkers, the most common are those having photoreactive groups that become reactive fluorophores when activated by UV light thereby resulting in photolabeling the cross-linked moieties.[ PubMed:10679368 PubMed:7708014 ]
Also Known As"bifunctional agent crosslink" ; "Label transfer techniques" ; "Photoaffinity labelling"
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 protein cross-linking with a bifunctional reagent (all annotations which use CV term, excluding "NOT" statements)
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  biochemical
   |__cross-linking study
       |__protein cross-linking with a bifunctional reagent  91 rec.
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  • "bifunctional agent crosslink" EXACT PSI-MI-short
    "Label transfer techniques" EXACT PSI-MI-alternate
    "Photoaffinity labelling" EXACT PSI-MI-alternate
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