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General Information
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| Term |
protein cross-linking with a bifunctional reagent |
ID (Ontology) |
MI:0031 (Molecular Interactions) |
| Definition |
Cross-linking agents induce the formation of covalent bonds among proteins that are neighbours. The cross-linker may be a bifunctional molecule having two reactive ends linked by a spacer, often containing a disulfide bond. When a reducing agent is added the disulfide bridge is cleaved, the cross-linked pairs are released and can be identified. There are various classes of cross-linkers, the most common are those having photoreactive groups that become reactive fluorophores when activated by UV light thereby resulting in photolabeling the cross-linked moieties.[ PubMed:10679368 PubMed:7708014 ] |
| Also Known As |
"bifunctional agent crosslink" ; "Label transfer techniques" ; "Photoaffinity labelling" |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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protein cross-linking with a bifunctional reagent (all annotations which use CV term, excluding "NOT" statements) | 91 |
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Relationships