FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
CV Term Report
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Term ubiquitin reconstruction ID (Ontology) MI:0112 (Molecular Interactions)
Definition The split-ubiquitin system provides a method for examining the interactions of membrane proteins. In the split-ubiquitin system, two integral membrane proteins to be studied are fused to two different ubiquitin moieties: a C-terminal ubiquitin moiety (\"Cub\", residues 35-76) and an N-terminal ubiquitin moiety (\"Nub\", residues 1-34). These fused proteins are called the bait and prey, respectively. In addition to being fused to an integral membrane protein, the Cub moiety is also fused to a transcription factor that can be cleaved off by ubiquitin specific proteases. Upon bait-prey interaction, Nub and Cub-moieties assemble, reconstituting the split-ubiquitin. The reconstituted split-ubiquitin molecule is recognized by ubiquitin specific proteases, which cleave off the reporter protein, allowing it to induce the transcription of reporter genes.[ PubMed:9560251 ]
Also Known As "ub reconstruction"
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 ubiquitin reconstruction (all annotations which use CV term, excluding "NOT" statements)       4
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  transcriptional complementation assay
   |__membrane yeast two hybrid
       |__ubiquitin reconstruction  4 rec.
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Is a membrane yeast two hybrid
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Synonyms
  • "membrane yeast two-hybrid" RELATED
    "myth" RELATED
    "split-ubiquitin" RELATED
    "ub reconstruction" EXACT PSI-MI-short
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