Allele Dmel\dsx²³

General Information
Symbol	Dmel\dsx²³	Species	D. melanogaster
Name		FlyBase ID	FBal0003176
Feature type	allele	Associated gene	Dmel\dsx
Also Known As	dsx^d+r3

Allele class	loss of function allele
Mutagen	X ray
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Taylor et al., 1994)
Mutagen	X ray (Baker and Wolfner, 1988, Baker et al., 1991)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	dsx^D (Duncan and Kaufman, 1975, Baker and Wolfner, 1988, Baker et al., 1991)
Nature of the lesion	Statement Reference Produces a shortened transcript. (An and Wensink, 1995)
Caused by aberration	In(3R)dsx²³ (Baker et al., 1991, Duncan and Kaufman, 1975, Kaufman et al., 1980, Taylor et al., 1994, An and Wensink, 1995, Villella and Hall, 1996, Billeter et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective \| male \| recessive (Villella and Hall, 1996) neuroanatomy defective (Billeter et al., 2006)
Phenotype Manifest In
	eye (Duncan and Kaufman, 1975) female genital disc (with Df(3R)dsx15) (Keisman and Baker, 2001) gonad \| embryonic stage (with dsx¹) (DeFalco et al., 2004) gonadal sheath proper primordium \| female (with dsx¹) (DeFalco et al., 2003) hub cell (with dsx¹) (Le Bras and Van Doren, 2006) leg (Duncan and Kaufman, 1975) serotonergic neuron (Billeter et al., 2006)
Detailed Description
	Statement Reference dsx[23] mutants appear intersexual externally. Mutant males exhibit a decrease in male-specific serotonergic neurons, while females display ectopic serotonergic neurons. These neurons are differentially patterned depending on the sex of the mutant. In dsx[23] females, a single cluster of three to four serotonergic neurons develops either ventrally or dorsally. In males, both clusters develop but contain 50% fewer neurons than wild-type males. (Billeter et al., 2006) dsx[1]/dsx[23] mutant gonads are masculinised with respect to hub formation (germline stem cell niche formation). (Le Bras and Van Doren, 2006) dsx¹/dsx²³ mutant embryonic gonads appear fully masculinised. (DeFalco et al., 2004) In the somatic gonad, stage 15 male dsx¹/dsx²³ mutant embryos are indistinguishable from wild-type males. In contrast, the gonads of stage 15 female dsx¹/dsx²³ mutant embryos exhibit a completely masculinized phenotype, in which male-specific somatic gonadal precursors are clearly present. (DeFalco et al., 2003) Both the male and female genital primordia develop in XX genital discs. (Keisman and Baker, 2001) XY males show courtship sluggishness when compared to wild type siblings. Aging does not improve the courtship performance. Song pulses are similar to those of dsx⁺ males, though number and duration of song bouts are much reduced. No sine-song bouts whatsoever are generated by dsx mutants. In elicitation and rejection tests, dsx haplo-X mutants demonstrate an attractiveness that cannot be explained by general enfeeblement, such as inability to reject courtship advances. (Villella and Hall, 1996) XX dsx²³/Df(3R)dsx15 flies do not show any male-specific courtship when paired with mature virgin females. 100% of XY dsx²³/Df(3R)dsx15 flies show male-specific courtship when paired with mature virgin females. XY dsx²³/Df(3R)dsx15 flies have a reduced courtship index (compared to control males) when paired with immature male flies. XY dsx²³/Df(3R)dsx15 flies have an essentially normal courtship song compared to control XY flies. (Taylor et al., 1994) Sex specific neuroblasts fail to undergo any postembryonic divisions in male or female larval nervous systems. (Taylor and Truman, 1992) Dorsal musculature does not undergo sexual transformation. (Taylor, 1992) When crossed to Df(3R)dsx2D progeny are viable and display the dsx phenotype. (Baker et al., 1991) Homozygotes are intersexual. Adults have roughened eyes and deformed legs. (Duncan and Kaufman, 1975)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	1849 In(3R)dsx²³, dsx²³ Ubx^bx-1 sr¹ e^s/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer
	Revertant.
Comments
	Rank order for subnormal courtship in dsx alleles is dsx¹ < dsx¹⁶ < dsx¹⁵ < dsx²³. (Villella and Hall, 1996)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	dsx²³ (Lee et al., 2006, Le Bras and Van Doren, 2006, Billeter et al., 2006) dsx^d+r3 (Arbeitman et al., 2004, Piano et al., 1999, Lebo et al., 2009) dsx^D+R3 (Keisman and Baker, 2001, Cann et al., 2000, An and Wensink, 1995, Baker et al., 1991, Baker and Wolfner, 1988, Duncan and Kaufman, 1975, ) dsx^d+R3 (Lebo et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 19 )
Research paper	Lebo et al., 2009, BMC Genomics 10: 80 Somatic, germline and sex hierarchy regulated gene expression during Drosophila metamorphosis. [FBrf0207472] Billeter et al., 2006, Curr. Biol. 16(11): 1063--1076 Isoform-specific control of male neuronal differentiation and behavior in Drosophila by the fruitless gene. [FBrf0194977] Le Bras and Van Doren, 2006, Dev. Biol. 294(1): 92--103 Development of the male germline stem cell niche in Drosophila. [FBrf0190137] Lee et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(33): 12580--12585 Sex- and clock-controlled expression of the neuropeptide F gene in Drosophila. [FBrf0194171] Arbeitman et al., 2004, Development 131(9): 2007--2021 A genomic analysis of Drosophila somatic sexual differentiation and its regulation. [FBrf0174573] DeFalco et al., 2004, Mech. Dev. 121(11): 1323--1333 Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad. [FBrf0180482] DeFalco et al., 2003, Dev. Cell 5(2): 205--216 Sex-specific apoptosis regulates sexual dimorphism in the Drosophila embryonic gonad. [FBrf0162055] Keisman and Baker, 2001, Development 128(9): 1643--1656 The Drosophila sex determination hierarchy modulates wingless and decapentaplegic signaling to deploy dachshund sex-specifically in the genital imaginal disc. [FBrf0135727] Cann et al., 2000, Dev. Genes Evol. 210(4): 200--206 A new family of adenylyl cyclase genes in the male germline of Drosophila melanogaster. [FBrf0126917] Piano et al., 1999, Genetics 152(2): 605--616 Evidence for redundancy but not trans factor-cis element coevolution in the regulation of Drosophila Yp genes. [FBrf0109003] Villella and Hall, 1996, Genetics 143(1): 331--344 Courtship anomalies caused by doublesex mutations in Drosophila melanogaster. [FBrf0087763] An and Wensink, 1995, Genes Dev. 9(2): 256--266 Integrating sex- and tissue-specific regulation within a single Drosophila enhancer. [FBrf0079849] Taylor et al., 1994, Dev. Genet. 15(3): 275--296 Behavioral and neurobiological implications of sex-determining factors in Drosophila. [FBrf0074590] Taylor and Truman, 1992, Development 114: 625--642 Commitment of abdominal neuroblasts in Drosophila to a male or female fate is dependent on genes of the sex-determining hierarchy. [FBrf0055884] Taylor, 1992, Genetics 132(1): 179--191 Differentiation of a male-specific muscle in Drosophila melanogaster does not require the sex-determining genes doublesex or intersex. [FBrf0056208] Baker et al., 1991, Genetics 127: 125--138 The doublesex locus of Drosophila melanogaster and its flanking regions: a cytogenetic analysis. [FBrf0054129] Baker and Wolfner, 1988, Genes Dev. 2: 477--489 A molecular analysis of doublesex, a bifunctional gene that controls both male and female sexual differentiation in Drosophila melanogaster. [FBrf0048115] Kaufman et al., 1980, Genetics 94(1): 115--133 Cytogenetic analysis of chromosome 3 in Drosophila melanogaster: the homoeotic gene complex in polytene chromosome interval 84A-B. [FBrf0034841] Duncan and Kaufman, 1975, Genetics 80: 733--752 Cytogenetic analysis of chromosome 3 in Drosophila melanogaster: mapping of the proximal portion of the right arm. [FBrf0027527]

Allele Dmel\dsx23

Allele Dmel\dsx²³