FB2025_01 , released February 20, 2025
Allele: Dmel\en58
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General Information
Symbol
Dmel\en58
Species
D. melanogaster
Name
FlyBase ID
FBal0003773
Feature type
allele
Associated gene
Dmel\en
Associated Insertion(s)
Carried in Construct
Also Known As
enIM99, enIM199, enIM
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Andrew et al., 1997, Dittrich et al., 1997)
Mutagen
ethyl methanesulfonate
(Condie and Brower, 1989)
Progenitor genotype
Cytology

Polytene chromosomes normal.

(Condie and Brower, 1989)
Description

Amino acid replacement: Q497term.

(Gustavson et al., 1996, Heemskerk et al., 1991)

The premature stop codon is predicted to truncate the protein upstream of the DNA binding motif.

(Heemskerk et al., 1991)

Point mutant.

(Williams et al., 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:11,524,769..11,524,769 [-]
Nucleotide change:

C11524769T

Reported nucleotide change:

C?T

Amino acid change:

Q497term | en-PA; Q497term | en-PB

Reported amino acid change:

Q497term

(Gustavson et al., 1996, Heemskerk et al., 1991)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      enJ86/en58 flies survive with slight defects in the posterior wing compartment.

      (Kwon et al., 2009)

      22% of Df(2R)enΔ530/en58 wings exhibit split veins in the posterior compartment.

      (DeVido et al., 2008)

      Double mutant phenotype with CrebA mutants is additive.

      (Andrew et al., 1997)

      Mutants exhibit pairwise fusion of the abdominal segments, failure of head involution and extensive cell death causing a shortened cuticle.

      (Whiteley and Kassis, 1997)

      Strong allele.

      (Gustavson et al., 1996)

      No reduction in rate of germ band extension, in wg, ptc, en triple mutants.

      (Irvine and Wieschaus, 1994)

      Mutant embryos have a lawn of row-2, row-3, row-4, row-5 and row-6-type ventral denticles, with naked patches in alternate segments.

      (Bejsovec and Wieschaus, 1993)

      hh expression fades prematurely in mutant embryos.

      (Lee et al., 1992)

      Heterozygotes are phenotypically wild type.

      (Heemskerk et al., 1991)

      Weak allele.

      (Peifer and Wieschaus, 1990)

      en1/en58 transheterozygotes have a variable phenotype. About one quarter are nearly indistinguishable from wild-type, while the rest have interruptions of varying extent in the fourth vein. en2/en58 transheterozygotes survive to larvae or pharate adults in a few cases. The pharate adults have severe abnormalities in distal segments of all legs and extreme posterior abnormalities in the wing.

      (Condie and Brower, 1989)

      en58/en1 flies have wild-type shape wings and a wild-type scutellum. Vein III appears thickened and tends to have a posteriorwards bulge about halfway along its length. The anterior crossvein (ACV) is often reduced or absent. Small plexae of vein tissue tend to occur close to the ACV and the posterior crossvein. Vein IV is frequently interrupted and often carries campaniform sensilla. Bristles are occasionally seen in the posterior row, but they never have the characteristics of the middle triple row.

      (Gubb, 1985)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      en58 has embryonic segment phenotype, enhanceable by inv12A-11

      (Whiteley and Kassis, 1997)
      Suppressed by
      Statement
      Reference

      en58 has embryo phenotype, suppressible by Agam\enen-12A-5R

      (Whiteley and Kassis, 1997)

      en58 has embryo phenotype, suppressible by Agam\enen-10B-5

      (Whiteley and Kassis, 1997)
      NOT suppressed by
      Statement
      Reference

      en58 has phenotype, non-suppressible by eRF1[+]/eRF1F2

      (Chao et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Agam\enen-12A-5R and Agam\enen-10B-5 rescue causes slight defects in the posterior compartments of the wings, both sexes are fertile.

      (Whiteley and Kassis, 1997)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      References (20)