FlyBase Allele Report: Dmel\tko[3]

General Information

Symbol

Dmel\tko³

Species

D. melanogaster

Name

FlyBase ID

FBal0016809

Feature type

allele

Associated gene

Dmel\tko

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Short insertion/deletion at codon 108-109, resulting in a frameshift and a premature stop codon.

(Toivonen et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:2,442,461..2,442,462 [-]

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement: Short insertion/deletion at codon 108-109, (V108 is GTA, T and A are deleted, see Fig 6) resulting in a frameshift and a premature stop codon.

(Toivonen et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Kemppainen et al., 2009)

abnormal developmental rate (with tko^25t)

(Toivonen et al., 2003)

abnormal developmental rate (with tko^25t), with tko^25t.tTa

(Toivonen et al., 2003)

abnormal neuroanatomy | adult stage | somatic clone

(Chihara et al., 2007)

bang sensitive

(Kemppainen et al., 2009)

bang sensitive (with tko^25t), with tko^25t.tTa

(Toivonen et al., 2003)

lethal

lethal | recessive

(Toivonen et al., 2001)

lethal - all die before end of larval stage

(Toivonen et al., 2003)

partially lethal - majority die (with tko^25t)

(Toivonen et al., 2003)

partially lethal - majority die (with tko^25t), with tko^25t.tTa

(Toivonen et al., 2003)

short lived (with tko^25t), with tko^25t.tTa

(Toivonen et al., 2003)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | adult stage | somatic clone | somatic clone

(Chihara et al., 2007)

gamma Kenyon cell

(Chihara et al., 2007)

Detailed Description

Statement

Reference

tko³ mutants exhibit a delay in eclosion and an increase in recovery time after vortexing (bang sensitivity).

(Kemppainen et al., 2009)

Adult DL1 projection neuron homozygous single-cell clones target their dendrites correctly to the DL1 glomerulus and show considerable dendritic elaboration. However, the dendritic density in the glomerulus is reduced compared to wild type in 2-5 day old adults. In 30 day old adults, mutant DL1 projection neuron dendrites no longer occupy the centre of the DL1 glomerulus and instead some dendrites surround the glomerulus while others are outside of the antennal lobes. Axonal growth, guidance and terminal branching appears largely normal in anterodorsal projection neuron homozygous single-cell clones.

Adult mushroom body γ neuron single-cell homozygous clones show a mild reduction in the number of dendritic branches and dendritic claws compared to wild type. The number of short terminal axonal branches is normal in the mutant cells, while there is a significant increase in the number of long terminal axonal branches compared to wild type.

(Chihara et al., 2007)

Mutants do not display a dominant Minute bristle phenotype.

(Marygold et al., 2007)

tko³ homozygous females and hemizygous males are larval lethal and arrest development as first or second instar larvae. tko³/tko^25t transheterozygous females are able to complete development but with an extremely long delay and majority fail to eclose successfully. The adults that manage to eclose are extremely lethargic, attempts at bang-sensitivity measurements lead to prolonged or permanent paralysis and most die shortly after eclosion.

tko³/tko^25t transheterozygotes carrying additional one or three copies of the tko^25t.tTa mutant allele on a transgene improved the phenotypic defects of the transheterozygotes: the developmental delay is reduced, greater fraction of the flies manage to eclose, the adults show long but measurable recovery times in the bang-sensitivity assay and greatly reduced adult lifespan.

(Toivonen et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

tko³ has abnormal developmental rate phenotype, non-suppressible by Dp(1)weeb¹

(Kemppainen et al., 2009)

tko³ has bang sensitive phenotype, non-suppressible by Dp(1)weeb¹

(Kemppainen et al., 2009)

tko³ has abnormal developmental rate phenotype, non-suppressible by Dp(1)weeb²

(Kemppainen et al., 2009)

tko³ has bang sensitive phenotype, non-suppressible by Dp(1)weeb²

(Kemppainen et al., 2009)

tko³ has abnormal developmental rate phenotype, non-suppressible by Dp(1)weeb³

(Kemppainen et al., 2009)

tko³ has bang sensitive phenotype, non-suppressible by Dp(1)weeb³

(Kemppainen et al., 2009)

tko^25t.tTa, tko³ has abnormal developmental rate phenotype, non-suppressible by Dp(1)weeb¹

(Kemppainen et al., 2009)

tko^25t.tTa, tko³ has bang sensitive phenotype, non-suppressible by Dp(1)weeb¹

(Kemppainen et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A Dp(1)weeb¹, Dp(1)weeb² or Dp(1)weeb³ background gives a female phenotype that is still completely mutant, both for developmental delay and bang sensitivity.

Dp(1)weeb¹/tko³ mutants retain a developmental delay even when an expressed, ectopic copy of tko^25t.tTa is also present.

(Kemppainen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tko³ is rescued by tko^25t-H85L.tTa

(Toivonen et al., 2001)

Not rescued by

tko³ is not rescued by tko^25t.tTa

(Toivonen et al., 2001)