Allele Dmel\per^{BG.T:Ppyr\LUC}

General Information
Symbol	Dmel\per^{BG.T:Ppyr\LUC}	Species	D. melanogaster
Name		FlyBase ID	FBal0063147
Feature type	allele	Associated gene	Dmel\per

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Stanewsky et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Fusion of a 9.8kb BamHI per fragment extending from -4200 to +5627 to a 1.8kb BamHI-KpnI Ppyr\LUC cDNA. Expression is influenced by 1kb EcoRI fragment carring the SV40 polyadenylation site. Two thirds of the per protein is fused in frame to the Ppyr\LUC cDNA. (Stanewsky et al., 1997)
Carried in construct	P{BG-luc} (Glaser and Stanewsky, 2005, Wuelbeck et al., 2005, Giebultowicz et al., 2000, Kaneko et al., 2000, Stanewsky et al., 1998, Belvin et al., 1999, Helfrich-Forster et al., 2001, Krishnan et al., 2001, Stanewsky et al., 1997, Yuan et al., 2005, Koh et al., 2006, Dolezelova et al., 2007, Zheng et al., 2007, Hung et al., 2009, Sehadova et al., 2009, Stanewsky et al., 2002)
Tagged with	T:Ppyr\LUC (Stanewsky et al., 1998, Belvin et al., 1999, Giebultowicz et al., 2000, Kaneko et al., 2000, Krishnan et al., 2001, Helfrich-Forster et al., 2001, Glaser and Stanewsky, 2005, Wuelbeck et al., 2005, Stanewsky et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor rhythm defective (Glaser and Stanewsky, 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies expressing per^{BG.T:Ppyr\LUC} are able to entrain to a 12 hour:12 hour LD cycle at 25^oC. These flies entrain to a 12 hour:12 hour 25^oC:18^oC temperature cycle in LL but exhibit maximum activity during the middle of the warm phase, while the wild-type activity peak is at the end of the warm phase and extends into the phase. per^{BG.T:Ppyr\LUC} flies can also entrain to two consecutive warm:cold cycles in LL where the second cycle is phase advanced by 6 hours; they reach a new stable phase relationship after 3-4 days by steadily advancing their average activity peak during the warm phase. (Glaser and Stanewsky, 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference nocte¹, per^{BG.T:Ppyr\LUC} has locomotor behavior defective phenotype (Glaser and Stanewsky, 2005, Glaser and Stanewsky, 2005)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Flies expressing per^{BG.T:Ppyr\LUC} in a nocte¹ background are able to entrain to a 12 hour:12 hour LD cycle at 25^oC. In contrast to wild-type flies, per^{BG.T:Ppyr\LUC} nocte¹ flies fail to entrain to a 12 hour:12 hour 25^oC:18^oC temperature cycle in LL; these flies are constantly active throughout both temperatures, except for a drop in activity during a ~1 hour window shortly after transition to the cold phase. Activity levels are higher in the warm phase. Further, the flies fail to entrain to two consecutive warm:cold cycles in LL where the second cycle is phase advanced by 6 hours, instead they abruptly change their activity level at the time of temperature transition. The flies behave similarly to control flies when kept in constant darkness at a variety of constant temperatures. (Glaser and Stanewsky, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	LUC::per^BG per^{BG.T:Ppyr\LUC}
Name Synonym
Secondary FlyBase IDs

References ( 16 )
Research paper	Hung et al., 2009, J. Biol. Rhythms 24(3): 183--192 HSP90, a Capacitor of Behavioral Variation. [FBrf0207993] Sehadova et al., 2009, Neuron 64(2): 251--266 Temperature entrainment of Drosophila's circadian clock involves the gene nocte and signaling from peripheral sensory tissues to the brain. [FBrf0209187] Dolezelova et al., 2007, Genetics 177(1): 329--345 Rhythm defects caused by newly engineered null mutations in Drosophila's cryptochrome gene. [FBrf0201773] Zheng et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(40): 15899--15904 FOXO and insulin signaling regulate sensitivity of the circadian clock to oxidative stress. [FBrf0216135] Koh et al., 2006, Science 312(5781): 1809--1812 JETLAG resets the Drosophila circadian clock by promoting light-induced degradation of TIMELESS. [FBrf0193019] Glaser and Stanewsky, 2005, Curr. Biol. 15(15): 1352--1363 Temperature synchronization of the Drosophila circadian clock. [FBrf0187310] Wuelbeck et al., 2005, Genetics 169(2): 751--766 The novel Drosophila tim(blind) mutation affects behavioral rhythms but not periodic eclosion. [FBrf0187663] Yuan et al., 2005, Neuron 47(1): 115--127 Serotonin modulates circadian entrainment in Drosophila. [FBrf0188183] Stanewsky et al., 2002, J. Biol. Rhythms 17(4): 293--306 Mapping of elements involved in regulating normal temporal period and timeless RNA expression patterns in Drosophila melanogaster. [FBrf0151362] Helfrich-Forster et al., 2001, Neuron 30(1): 249--261 The circadian clock of fruit flies is blind after elimination of all known photoreceptors. [FBrf0135910] Krishnan et al., 2001, Nature 411(6835): 313--317 A new role for cryptochrome in a Drosophila circadian oscillator. [FBrf0135900] Giebultowicz et al., 2000, Curr. Biol. 10(2): 107--110 Transplanted Drosophila excretory tubules maintain circadian clock cycling out of phase with the host. [FBrf0125137] Kaneko et al., 2000, J. Neurobiol. 43(3): 207--233 Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms. [FBrf0128517] Belvin et al., 1999, Neuron 22(4): 777--787 The Drosophila dCREB2 gene affects the circadian clock. [FBrf0108141] Stanewsky et al., 1998, Cell 95(5): 681--692 The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila. [FBrf0105937] Stanewsky et al., 1997, EMBO J. 16(16): 5006--5018 Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila. [FBrf0096339]

Allele Dmel\perBG.T:Ppyr\LUC

Allele Dmel\per^{BG.T:Ppyr\LUC}