Allele Dmel\Sec61α^{Scer\UAS.cKa.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Sec61α^{Scer\UAS.cKa.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0152192
Feature type	allele	Associated gene	Dmel\Sec61α

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Kanuka et al., 2003) in vitro construct - regulatory fusion (Kanuka et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS drives expression of Sec61α tagged with T:Zzzz\FLAG. (Kanuka et al., 2003)
Carried in construct	P{UAS-Sec61α.FLAG} (Kanuka et al., 2003, Arsham and Neufeld, 2009)
Tagged with	T:Zzzz\FLAG (Kanuka et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	autophagic vacuole & larval fat body \| somatic clone \| cell autonomous, with Scer\GAL4^Act5C.PP (Arsham and Neufeld, 2009) lysosome & larval fat body \| somatic clone \| cell autonomous, with Scer\GAL4^Act5C.PP (Arsham and Neufeld, 2009)
Detailed Description
	Statement Reference Clonal expression of Sec61α[Scer\UAS.cKa.T:Zzzz\FLAG] in fat bodies (generated using Scer\GAL4[Act5C.PP]) supresses starvation-induced accumulation of lysosomes and results in fewer and smaller Atg8a-containing autophagosomes. (Arsham and Neufeld, 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells. (Kanuka et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Sec61α^{Scer\UAS.cKa.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Arsham and Neufeld, 2009, PLoS ONE 4(6): e6068 A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway. [FBrf0208155] Kanuka et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(20): 11723--11728 Cytosol-endoplasmic reticulum interplay by Sec61α translocon in polyglutamine-mediated neurotoxicity in Drosophila. [FBrf0173066]

Allele Dmel\Sec61αScer\UAS.cKa.T:Zzzz\FLAG

Allele Dmel\Sec61α^{Scer\UAS.cKa.T:Zzzz\FLAG}