FlyBase Allele Report: Dmel\par-1[T786A.N1S.UASp.mGFP6]

General Information

Symbol

Dmel\par-1^{T786A.N1S.UASp.mGFP6}

Species

D. melanogaster

Name

FlyBase ID

FBal0244709

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

Carried in Construct

P{UASp-par-1.N1S.T786A.GFP}

Key Links

Transgenic product class

missense_variant

(Doerflinger et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Doerflinger et al., 2010)

Mutagen

in vitro construct

(Doerflinger et al., 2010)

Progenitor genotype

par-1^{N1S.UASp.mGFP6}

(Doerflinger et al., 2010)

Carried in construct

P{UASp-par-1.N1S.T786A.GFP}

Cytology

Description

The T786A mutation has been introduced into the par-1 coding region in par-1^{N1S.UASp.mGFP6}.

(Doerflinger et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

par-1

Tagged with

mGFP6

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

microtubule, with Scer\GAL4^unspecified

(Doerflinger et al., 2010)

microtubule | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

oocyte nucleus, with Scer\GAL4^unspecified

(Doerflinger et al., 2010)

Detailed Description

Statement

Reference

Embryos from mothers expressing par-1^{T786A.N1S.Scer\UAS.P\T.T:Avic\GFP-m6} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} exhibit abnormal centrosome location in cells of the epithelium, and enhances centrosomal microtubule asters, but does not have an apparent effect on basolateral microtubule bundles.

(Jiang et al., 2015)

Expression of par-1^{T786A.N1S.Scer\UAS.P\T.T:Avic\GFP-m6} under the control of Scer\GAL4^unspecified during oogenesis disrupts oocyte polarity; the nucleus is never localised to the dorsal/anterior corner of the oocyte as in wild type, but is instead mislocalised to the centre of the cell. Prior to the repolarisation of the oocyte, there is no effect on the organisation of the microtubules. However, at stage 9, the microtubule organisation is completely disrupted in the mutant oocytes; all cortex-associated microtubules appear to be missing.

(Doerflinger et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

par-1^{T786A.N1S.Scer\UAS.P\T.T:Avic\GFP-m6}

par-1^{T786A.N1S.UASp.mGFP6}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close