FB2025_01 , released February 20, 2025
Allele: Dmel\Mt2genTG.EGFP
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General Information
Symbol
Dmel\Mt2genTG.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0246945
Feature type
allele
Associated gene
Dmel\Mt2
Associated Insertion(s)
Carried in Construct
P{Mt2.genTG.EGFP}
Key Links
Transgenic product class
genomic_DNA
(Schaefer et al., 2008)
wild_type
(Schaefer et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Schaefer et al., 2008)
wild_type
(Schaefer et al., 2008)
Mutagen
in vitro construct
(Schaefer et al., 2010)
Progenitor genotype
Carried in construct
P{Mt2.genTG.EGFP}
Cytology
Description

Genomic fragment containing Mt2 which has been tagged with EGFP.

(Schaefer et al., 2010)

Genomic fragment that encompasses 300bp upstream of the Mt2 start site and 150bp downstream of the stop codon.

(Schaefer et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
Mt2
Encoded product / tool
Mt2
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 1 -- 17
      organism | ubiquitous
      (Schaefer et al., 2008)
      embryonic stage 2 -- 6
      cell

      Comment: interphase

      (Schaefer et al., 2008)
      cell

      Comment: mitosis

      (Schaefer et al., 2008)
      third instar larval stage
      embryonic/larval salivary gland
      (Schaefer et al., 2008)
      adult stage | female
      ovary
      (Schaefer et al., 2008)
      ovary
      • nucleus
      (Schaefer et al., 2008)
      adult stage | male
      testis

      Comment: NOT hub cell of testis, NOT spermatozoon

      (Schaefer et al., 2008)
      testis
      • nucleus

      Comment: NOT hub cell of testis, NOT spermatozoon

      (Schaefer et al., 2008)
      adult stage | female & oogenesis
      ovary
      (Schaefer et al., 2010)
      Additional Information
      Statement
      Reference

      The Mt2genTG.T:AvicGFP-EGFP protein is enriched in the cytoplasm of ovaries in a homogenous distribution. Its distribution changes to a granular pattern upon heat shock.

      (Schaefer et al., 2010)

      The Mt2genTG.T:AvicGFP-EGFP protein is ubiquitously expressed throughout development, including most stages of gametogenesis, but not in mature sperm or the hub cell of the testis.

      (Schaefer et al., 2008)

      At the subcellular level, the Mt2genTG.T:AvicGFP-EGFP protein is mostly cytoplasmic, with some present in the nucleus. In early embryos, the cytoplasmic enrichment of Mt2genTG.T:AvicGFP-EGFP occurs at interphase, but the protein redistributes around nuclear structures during mitosis.

      (Schaefer et al., 2008)
       
      Marker for
      Reflects expression of
      Mt2
      (Schaefer et al., 2010, Schaefer et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Mt2genTG.EGFP rescues Mt299

      (Durdevic et al., 2013)
      Comments

      Expression of Mt2genTG.T:Avic\GFP-EGFP rescues the increased lethality seen in Mt299 mutant flies in response to Drosophila C Virus(DCV) infection.

      (Durdevic et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Dnmt2genTG-EGFP
      (Schaefer et al., 2010)
      Mt2genTG.EGFP
      Mt2genTG.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)