FB2025_01 , released February 20, 2025
Allele: Dmel\Rab3-GAPUAS.ECFP
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General Information
Symbol
Dmel\Rab3-GAPUAS.ECFP
Species
D. melanogaster
Name
FlyBase ID
FBal0258135
Feature type
allele
Associated gene
Dmel\Rab3-GAP
Associated Insertion(s)
Carried in Construct
P{UAS-ECFP-Rab3-GAP}
Also Known As
UAS-CFP-Rab3-GAP
Key Links
Transgenic product class
cDNA
(Müller et al., 2011)
wild_type
(Müller et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Müller et al., 2011)
wild_type
(Müller et al., 2011)
Mutagen
in vitro construct
(Müller et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-ECFP-Rab3-GAP}
Cytology
Description

UASt sequences regulate expression of the full-length open reading frame of Rab3-GAP isoform A (amplified from the LD40982 cDNA clone). The coding sequence is tagged at the N-terminal end with ECFP.

(Müller et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rab3-GAP
Tagged with
ECFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of rab3-GAPScer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4elav-C155 in a wild-type background does not affect synaptic homeostasis although there is a slight, statistically significant decrease in evoked neurotransmitter release.

      (Müller et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/Rab3-GAPUAS.ECFP rescues Rab3-GAPc04953

      (Müller et al., 2011)
      Comments

      A homeostatic increase in presynaptic quantal content is observed following application of PhTX to rab3-GAPc04953 mutants expressing rab3-GAPScer\UAS.T:Avic\GFP-YFP.Venus pre-synaptically under the control of Scer\GAL4elav-C155. The observed increase in quantal content offsets the PhTX-dependent decrease in mEPSP amplitude and restores EPSP amplitudes to baseline values.

      (Müller et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Rab3-GAPScer\UAS.T:Avic\GFP-YFP.Venus
      Rab3-GAPUAS.ECFP
      Rab3-GAPUAS.Venus
      rab3-GAPScer\UAS.T:Avic\GFP-YFP.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (2)