FB2025_01 , released February 20, 2025
Allele: Dmel\numbS2D.UAS.Tag:HA
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General Information
Symbol
Dmel\numbS2D.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0276717
Feature type
allele
Associated gene
Dmel\numb
Associated Insertion(s)
Carried in Construct
P{UAS-numb.S2D.HA}
Key Links
Transgenic product class
missense_variant
(Haenfler et al., 2012)
polypeptide_post_translational_processing_variant
(Haenfler et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Haenfler et al., 2012)
polypeptide_post_translational_processing_variant
(Haenfler et al., 2012)
Mutagen
in vitro construct
(Haenfler et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-numb.S2D.HA}
Cytology
Description

UASt regulatory sequences drive expression of a phosphomimetic form of numb (the two aPKC phosphorylation sites at residues 48 and 52 have been mutated from S to D) which is tagged with a Tag:HA epitope.

(Haenfler et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
numb
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of numbS2D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA results in the complete loss of type II neuroblasts prematurely in larval brains.

      Expression of numbS2D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4sca.PU results in a decreased number of scutellar bristles compared to wild type.

      (Haenfler et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The formation of supernumerary type I neuroblasts which is seen in l(2)gl334/l(2)gl3644 larval brains is suppressed by expression of numbS2D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ase.neuro.

      The formation of supernumerary type I neuroblasts which is seen in the brains of larvae expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA is suppressed by co-expression of numbS2D.Scer\UAS.T:Ivir\HA1.

      (Haenfler et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      numbS2D.Scer\UAS.T:Ivir\HA1
      numbS2D.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)