Expression of Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf significantly reduces the response of the M pacemaker neurons in the brain to Pdf, while the response to either Dh31 or dopamine is unaffected.
Expression of Ac3Scer\UAS.cDa under the control of Scer\GAL4Mai179 does not significantly alter the response of the E pacemaker neurons in the brain to Pdf.
Flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf show a very strong reduction in the morning anticipation peak of circadian locomotor behaviour compared to wild-type flies under light-dark conditions. Under constant darkness conditions, 83% of the flies are arrhythmic.
Ac3UAS.cDa, Scer\GAL4P2.4.Pdf has abnormal locomotor rhythm phenotype, suppressible | partially by PdfrUAS.cMa, Scer\GAL4P2.4.Pdf
Co-expression of PdfrScer\UAS.cMa fully rescues while co-expression of PdfrT:Hsap\MYC partially rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf.
Co-expression of Dh31-R1Scer\UAS.cDa partially rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf.
Co-expression of PdfrScer\UAS.cMa slightly reduces the arrhythmicity in constant darkness conditions which is seen in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf. However, the loss of morning anticipation which is seen in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf under light-dark conditions is not rescued by co-expression of PdfrScer\UAS.cMa.
The reduced response of M pacemaker neurons in the brain to Pdf seen in flies expressing Ac3JF03041 under the control of Scer\GAL4P2.4.Pdf is rescued by co-expression of Ac3Scer\UAS.cDa when flies are raised at 25[o]C and then moved to 18[o]C as adults 12-15 hours before testing.
