FB2025_01 , released February 20, 2025
Allele: Dmel\TBPHA405T.UAS.mRFP1
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General Information
Symbol
Dmel\TBPHA405T.UAS.mRFP1
Species
D. melanogaster
Name
FlyBase ID
FBal0277058
Feature type
allele
Associated gene
Dmel\TBPH
Associated Insertion(s)
Carried in Construct
P{UAS-TBPH.A405T.RFP}
Key Links
Transgenic product class
missense_variant
(Estes et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Estes et al., 2011)
Mutagen
in vitro construct
(Estes et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-TBPH.A405T.RFP}
Cytology
Description

UAS regulatory sequences drive expression of a mutant form of TBPH in which the alanine at amino acid 405 has been replaced by threonine. The construct is tagged at the N-terminal end with mRFP1 fluorescent protein.

(Estes et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
TBPH
Tagged with
mRFP1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis
      CEA
      (Estes et al., 2011)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TBPHA405T.Scer\UAS.T:Disc\RFP in the eye under the control of Scer\GAL4GMR.PF results in severe eye depigmentation and neurodegeneration at 25[o]C.

      Expression of TBPHA405T.Scer\UAS.T:Disc\RFP under the control of Scer\GAL4D42 has no effect on the number of boutons in the larval neuromuscular junction. The number of synaptic vesicles is reduced compared to wild type and supernumerary satellite boutons are not present. The number of axonal branches is also comparable with wild type.

      Expression of TBPHA405T.Scer\UAS.T:Disc\RFP in motor neurons under the control of Scer\GAL4D42 results in locomotor defects in third instar larvae. Crawling larvae rolled ventral side up take longer to turn back to dorsal side up.

      Expression of TBPHA405T.Scer\UAS.T:Disc\RFP under the control of Scer\GAL4D42 results in a modest amount of motor neuron apoptosis in the larval ventral ganglia.

      (Estes et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TBPHA405T.Scer\UAS.T:Disc\RFP
      TBPHA405T.UAS.mRFP1
      Name Synonyms
      Secondary FlyBase IDs
        References (2)