FlyBase Allele Report: Dmel\milt[33-853]

General Information

Symbol

Dmel\milt^33-853

Species

D. melanogaster

Name

FlyBase ID

FBal0284439

Feature type

allele

Associated gene

Dmel\milt

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kitay et al., 2013)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Kitay et al., 2013)

lethal | recessive

(Kitay et al., 2013)

some die during first instar larval stage

(Kitay et al., 2013)

some die during second instar larval stage

(Kitay et al., 2013)

Phenotype Manifest In

adult antennal lobe | somatic clone

(Kitay et al., 2013)

adult olfactory receptor neuron | somatic clone

(Kitay et al., 2013)

mitochondrion

(Kitay et al., 2013)

mitochondrion | somatic clone | third instar larval stage

(Kitay et al., 2013)

motor neuron | somatic clone | third instar larval stage

(Kitay et al., 2013)

Detailed Description

Statement

Reference

Mutants show loss of anterograde mitochondria transport in motor neurons.

Homozygous olfactory receptor neuron (ORN) clones result in loss of ORN cell bodies and axons. Defects in the organisation of the antennal lobe neuropil, including loss of glomeruli is seen.

Homozygous single cell motor neuron clones in larvae show normal development and axon and synaptic morphology, but show loss of anterograde mitochondria transport.

Wallerian degeneration in an axotomized motor neuron within a crushed, but still continuous segmental nerve follows a similar time course in wild-type and milt^33-853 motor neuron clones, with continuity being maintained at 4 hours and a heterogeneous population of continuous and fragmented axons appearing 8 hours after crushing. A significantly smaller fraction of milt^33-853 axons are fragmented at 8 hours after crushing compared to wild-type axons, although all mutant and wild-type axons are fragmented by 16 hours after crushing.

(Kitay et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

milt^33-853 has abnormal neuroanatomy | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Or22a.7.717/Mmus\Wld^S.UAS

(Kitay et al., 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

milt^33-853 has adult olfactory receptor neuron | somatic clone phenotype, non-suppressible by Scer\GAL4^Or22a.7.717/Mmus\Wld^S.UAS

(Kitay et al., 2013)

milt^33-853 has adult antennal lobe | somatic clone phenotype, non-suppressible by Scer\GAL4^Or22a.7.717/Mmus\Wld^S.UAS

(Kitay et al., 2013)

milt^33-853 has mitochondrion | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Toll-6-D42/Nmnat^UAS.cZa

(Kitay et al., 2013)

milt^33-853 has motor neuron | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Toll-6-D42/Nmnat^UAS.cZa

(Kitay et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The anterograde mitochondria transport defects of single cell milt^33-853 larval motor neuron clones are not rescued by expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^D42.

Expression of either Nmnat^Scer\UAS.cZa or Nmnat^WR.Scer\UAS under the control of Scer\GAL4^elav-C155 protects both wild-type and milt^33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing.

(Kitay et al., 2013)

Xenogenetic Interactions

Statement

Reference

The loss of olfactory receptor neuron (ORN) cell bodies and axons and the morphological defects in the antennal lobe neuropil which are seen when homozygous milt^33-853 ORN clones are induced is not suppressed by expression of Mmus\wld^S.Scer\UAS under the control of Scer\GAL4^Or22a.7.717.

Expression of Mmus\wld^S.Scer\UAS under the control of Scer\GAL4^elav-C155 protects both wild-type and milt^33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing and delays Wallerian degeneration in both wild-type and milt^33-853 motor neuron clones 24 hours after crushing.

(Kitay et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

82424

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{Car20y}25F milt^33-853 P{neoFRT}40A/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

milt^33-853

milton^33-853

(Kitay et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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