FB2025_01 , released February 20, 2025
Allele: Dmel\FhosFL.UAS.EGFP
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General Information
Symbol
Dmel\FhosFL.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0295680
Feature type
allele
Associated gene
Dmel\Fhos
Associated Insertion(s)
Carried in Construct
M{UAS-EGFP-Fhos.FL}
Key Links
Transgenic product class
wild_type
(Lammel et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lammel et al., 2014)
Mutagen
in vitro construct
(Lammel et al., 2014)
Progenitor genotype
Carried in construct
M{UAS-EGFP-Fhos.FL}
Cytology
Description

UAS regulates expression of full-length Fhos (corresponding to isoform PA) tagged with EGFP.

(Lammel et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fhos
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4ptc.PU-mediated expression of FhosFL.Scer\UAS.T:Avic\GFP-EGFP does not affect the overall epithelial integrity or F-actin levels in wing discs.

      (Lammel et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Macrophages co-expressing FhosFL.Scer\UAS.T:Avic\GFP-EGFP and RokScer\UAS.P\T.T:Ivir\HA1 via Scer\GAL4Hml.Δ (restricted to late third instar larval stage using the Gal80ts system) show an increased number of stress fibers.

      Macrophages co-expressing FhosFL.Scer\UAS.T:Avic\GFP-EGFP and RokCA.Scer\UAS.P\T.T:Ivir\HA1 via Scer\GAL4Hml.Δ (restricted to late third instar larval stage using the Gal80ts system) show complete reorganization of actin filament bundles into a star-like pattern emerging from the cell center.

      (Lammel et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      FhosFL.UAS.EGFP/Scer\GAL4arm.PU rescues Df(3L)knittrigΔ1

      (Lammel et al., 2014)

      FhosFL.UAS.EGFP/Scer\GAL4Hml.Δ rescues Df(3L)knittrigΔ1

      (Lammel et al., 2014)
      Comments

      Scer\GAL4arm.PU-mediated expression of FhosFL.Scer\UAS.T:Avic\GFP-EGFP rescues both the lethality and wrinkled wing escaper phenotypes associated with Fhos deletion in Df(3L)knittrigΔ1 homozygotes.

      Scer\GAL4Hml.Δ-mediated expression of FhosFL.Scer\UAS.T:Avic\GFP-EGFP substantially rescues the cell spreading defects seen in Df(3L)knittrigΔ1 mutant macrophages.

      Scer\GAL4Hml.Δ-mediated expression of FhosFL.Scer\UAS.T:Avic\GFP-EGFP rescues the migration defects seen in macrophages of Df(3L)knittrigΔ1 mutants following a laser-induced wound in the pupal wing.

      (Lammel et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      FhosFL.Scer\UAS.T:Avic\GFP-EGFP
      FhosFL.UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)