FlyBase Allele Report: Dmel\RpL14[RNAi.UAS.RpL14-r]

General Information

Symbol

Dmel\RpL14^{RNAi.UAS.RpL14-r}

Species

D. melanogaster

Name

FlyBase ID

FBal0296444

Feature type

allele

Associated gene

Dmel\RpL14

Associated Insertion(s)

Carried in Construct

M{UAS-RpL14.RNAi.RpL14^r}

Key Links

Transgenic product class

RNAi_reagent

(Reeves et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Reeves et al., 2014)

Mutagen

in vitro construct

(Reeves et al., 2014)

Progenitor genotype

Carried in construct

M{UAS-RpL14.RNAi.RpL14^r}

Cytology

Description

This construct contains two elements. First, UAS regulates expression of a dsRNA corresponding to a 72bp non-contiguous region on exon 2 of RpL14, that targets endogenous RpL14 transcripts for RNAi-mediated knock-down. Second, a complete RpL14 genomic region (called "RpL14[r]") containing 14 synonymous mutations in exon 2 which confer insensitivity to the RNAi induced by the first element. The genomic region includes fragments of flanking genes to help ensure that all regulatory regions are included, and two intronic snoRNAs.

(Reeves et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

RpL14

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^Act5C.PI

(Reeves et al., 2014)

partially lethal - majority live, with Scer\GAL4^Act5C.PI

(Reeves et al., 2014)

viable, with Scer\GAL4^Act5C.PI

(Reeves et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^Act5C.PI, RpL14^{dsRNA.Scer\UAS.RpL14-r}/RpL14^{dsRNA.Scer\UAS.RpL14-r} "homozygous" flies are viable and do not show morphological abnormalities, prolonged development time, or abnormal egg-to-adult viability.

Scer\GAL4^Act5C.PI, RpL14^{dsRNA.Scer\UAS.RpL14-r}/+ "heterozygous" flies are viable and do not show morphological abnormalities - in particular, they do not exhibit short and thin scutellar bristles. However, these flies do exhibit a development time prolonged by ~20 hrs and the relative egg-to-adult viability is 20-50% lower than in "homozygous" flies.

(Reeves et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78575

w^*; P{Act5C-GAL4}25FO1/CyO; M{UAS-RpL14.RNAi.RpL14^r}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RpL14^{RNAi.UAS.RpL14-r}

RpL14^{dsRNA.Scer\UAS.RpL14-r}

RpL14^{dsRNA.UAS.RpL14-r}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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