FB2025_01 , released February 20, 2025
Allele: Dmel\mir-9asponge.UAS.EGFP
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General Information
Symbol
Dmel\mir-9asponge.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0299690
Feature type
allele
Associated gene
Dmel\mir-9a
Associated Insertion(s)
Carried in Construct
P{UAS-eGFP.mir-9a.sponge}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Loya et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-eGFP.mir-9a.sponge}
Cytology
Description

UASt regulatory sequences drive expression of a mir-9a "sponge" sequence, which consists of ten repetitive mir-9a complementary sequences with mismatches at positions 9-12 separated by a variable four-nucleotide linker. The sponge sequence is inserted into 3'UTR sequence downstream of the EGFP coding region, to enhance stability.

(Loya et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mir-9a
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Avic\GFPEGFP.Scer\UAS-mir-9aSP under the control of the ubiquitous Scer\GAL4αTub84B.PL driver results in posterior wing margin defects.

      Expression of mir-9asponge.Scer\UAS.T:Avic\GFP-EGFP under the control of the ubiquitous Scer\GAL4αTub84B.PL driver results in posterior wing margin defects. This phenotype is enhanced in a mir-9aJ22/+ background.

      (Loya et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      A mir-9aJ22/+ background enhances the wing margin defects seen when Avic\GFPEGFP.Scer\UAS-mir-9aSP is expressed under the control of Scer\GAL4αTub84B.PL.

      Expression of Avic\GFPEGFP.Scer\UAS-mir-9aSP along the dorsal-ventral boundary of the wing disc using Scer\GAL4vg.PM fails to rescue the wing margin phenotype seen in mir-9aJ22 homozygous mutants.

      (Loya et al., 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Avic\GFPEGFP.Scer\UAS.mir-9aSP
      mir-9asponge.Scer\UAS.T:Avic\GFP-EGFP
      mir-9asponge.UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0266091
      References (2)