UASp regulatory sequences drive expression of truncated spn-F sequences corresponding to residues 1-162 of spn-F, containing a coiled coil domain. Tagged at the N-terminal with GFP.
female sterile germline-dependent (with spn-F1), with Scer\GAL4VP16.mat.αTub67C
Scer\GAL4VP16.mat.αTub67C/spn-F1-162.UASp.N.GFP fails to rescue spn-F1
Expression of spn-F1-162.Scer\UAS.P\T.N.T:Avic\GFP in the germ line, under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 failed to rescue spn-F1-induced female sterility.