FlyBase Allele Report: Dmel\peb[RNAi.UAS.cPa]

General Information

Symbol

Dmel\peb^RNAi.UAS.cPa

Species

D. melanogaster

Name

FlyBase ID

FBal0318531

Feature type

allele

Associated gene

Dmel\peb

Associated Insertion(s)

Carried in Construct

P{UAS-peb.RNAi.P}

Also Known As

UAS-hntRNAi

Key Links

Transgenic product class

RNAi_reagent

(Pickup et al., 2009)

Mutagen

Nature of the Allele

Transgenic product class

RNAi_reagent

(Pickup et al., 2009)

Mutagen

Progenitor genotype

Carried in construct

P{UAS-peb.RNAi.P}

Cytology

Description

UASt regulatory sequences drive expression of an dsRNA targeted to a 0.7kb region of peb exon 3. It consists of a 1kb genomic peb fragment (0.3kb of the second intron and 0.7kb of exon 3) followed by a 0.7kb cDNA fragment which is in inverted orientation and corresponds to the 0.7kb exon 3 genomic fragment.

(Pickup et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

peb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

abnormal eye color, with Scer\GAL4^lz-gal4

(Pickup et al., 2009)

increased cell death, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

majority die during P-stage, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

melanotic necrosis, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

partially lethal - majority die, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

visible, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

visible, with Scer\GAL4^lz-gal4

(Pickup et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

eye, with Scer\GAL4^lz-gal4

(Pickup et al., 2009)

lens, with Scer\GAL4^GMR.PU

(Pickup et al., 2009)

Detailed Description

Statement

Reference

The majority of flies with peb^{dsRNA.Scer\UAS.cPa} (one copy) driven by Scer\GAL4^GMR.PU die in late pupal stages, with adult escapers: escapers have glossy eyes with mottled pigmentation, no well-defined facets, most facets lack intact lenses, and the eyes also have melanized necrotic patches on the surface. Similar but milder phenotypes are seen with peb^{dsRNA.Scer\UAS.cPa} (one copy) driven by Scer\GAL4^lz-gal4, with a gradient loss of pigmentation most severe at the posterior, and the eye has facets but is somewhat smoothened compared to wild type. When peb^{dsRNA.Scer\UAS.cPa} (one copy) is driven by Scer\GAL4^sv.PJ, there is no smoothening, loss of pigmentation, or necrosis.

(Pickup et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^lz-gal4, peb^RNAi.UAS.cPa has visible phenotype, suppressible | partially by Delta^UAS.GFP, Scer\GAL4^lz-gal4

(Pickup et al., 2009)

Scer\GAL4^lz-gal4, peb^RNAi.UAS.cPa has abnormal eye color phenotype, suppressible | partially by Delta^UAS.GFP, Scer\GAL4^lz-gal4

(Pickup et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^lz-gal4, peb^RNAi.UAS.cPa has eye phenotype, suppressible | partially by Delta^UAS.GFP, Scer\GAL4^lz-gal4

(Pickup et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dl^{Scer\UAS.T:Avic\GFP} partially suppresses the eye phenotypes seen in flies with peb^{dsRNA.Scer\UAS.cPa} driven by Scer\GAL4^lz-gal4 : eyes of rescued flies are less rough and have normal pigmentation.

(Pickup et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

peb^RNAi.UAS.cPa

peb^{dsRNA.Scer\UAS.cPa}

peb^{dsRNA.UAS.cPa}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close