FB2025_01 , released February 20, 2025
Allele: Hsap\APPAβ1-42.arctic.UAS.Tag:SS(nec)
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General Information
Symbol
Hsap\APPAβ1-42.arctic.UAS.Tag:SS(nec)
Species
H. sapiens
Name
FlyBase ID
FBal0318749
Feature type
allele
Associated gene
Hsap\APP
Associated Insertion(s)
Carried in Construct
P{UAS-Aβ1-42.arctic}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Burnouf et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Aβ1-42.arctic}
Cytology
Description

UASt regulatory sequences drive expression of the Aβ1-42 peptide carrying the 'arctic' mutation (E22G). The protein has been codon optimized for insect expression and also includes the Tag:SS(nec) secretion signal sequence.

(Burnouf et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\APP
Tagged with
Tag:SS(nec)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Hsap\APPAβ1-42.arctic.Scer\UAS.T:SS-nec driven by Scer\GAL4elav.Switch.PO in the presence of RU486 significantly reduces lifespan in adult flies compared to controls.

      (Burnouf et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Hsap\APPAβ1-42.arctic.UAS.Tag:SS(nec), Scer\GAL4elav.Switch.PO has short lived | RU486 conditional phenotype, non-enhanceable by tauKO/tauKO

      (Burnouf et al., 2016)
      NOT suppressed by
      Statement
      Reference

      Hsap\APPAβ1-42.arctic.UAS.Tag:SS(nec), Scer\GAL4elav.Switch.PO has short lived | RU486 conditional phenotype, non-suppressible by tauKO/tauKO

      (Burnouf et al., 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      tauKO/tauKO does not significantly modulate the shorter lifespan seen in flies with Hsap\APPAβ1-42.arctic.Scer\UAS.T:SS-nec driven by Scer\GAL4elav.Switch.PO in the presence of RU486.

      (Burnouf et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Aβ
      (Burnouf et al., 2016)
      Hsap\APPAβ1-42.arctic.Scer\UAS.T:SS-nec
      Hsap\APPAβ1-42.arctic.UAS.Tag:SS(nec)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)