FB2025_01 , released February 20, 2025
Allele: Dmel\Urm1ΔGG.UAS
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General Information
Symbol
Dmel\Urm1ΔGG.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0319061
Feature type
allele
Associated gene
Dmel\Urm1
Associated Insertion(s)
Carried in Construct
P{UAS-Urm1.ΔGG}
Key Links
Transgenic product class
dominant_negative_variant
(Khoshnood et al., 2016)
inframe_deletion
(Khoshnood et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Khoshnood et al., 2016)
inframe_deletion
(Khoshnood et al., 2016)
Mutagen
in vitro construct
(Khoshnood et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Urm1.ΔGG}
Cytology
Description

UASt regulatory sequences drive expression of Urm1 lacking the C-terminal di-glycine (GG) motif. This results in a dominant negative protein.

(Khoshnood et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Urm1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies with expression of Urm1ΔGG.Scer\UAS driven by Scer\GAL4Act5C.PU survive longer than controls under exposure to oxidative stress (10mM paraquat).

      (Khoshnood et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4Act5C.PU/Urm1ΔGG.UAS fails to rescue Urm1n123

      (Khoshnood et al., 2016)
      Comments

      Expression of Urm1ΔGG.Scer\UAS driven ubiquitously by Scer\GAL4Act5C.PU does not rescue lethality in Urm1n123/Urm1n123 animals; indeed, expression of Urm1ΔGG.Scer\UAS even increases lethality.

      (Khoshnood et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Urm1ΔGG.Scer\UAS
      Urm1ΔGG.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)