FB2025_01 , released February 20, 2025
Allele: Dmel\eglR.UASp.EGFP
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General Information
Symbol
Dmel\eglR.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0319852
Feature type
allele
Associated gene
Dmel\egl
Associated Insertion(s)
Carried in Construct
P{UASp-egl.R-GFP}
Key Links
Transgenic product class
wild_type
(Sanghavi et al., 2016)
Mutagen
    Nature of the Allele
    Transgenic product class
    wild_type
    (Sanghavi et al., 2016)
    Mutagen
    Progenitor genotype
    Carried in construct
    P{UASp-egl.R-GFP}
    Cytology
    Description

    UASp regulatory sequences drive expression of the egl coding sequence. Two silent nucleotide substitutions have been introduced into the coding region (A1599C and G1602C), so that the encoded transcript is not targeted by the shRNA produced by eglGL01170, but the resulting protein is wild-type in sequence. The protein is tagged at the C-terminal end with EGFP.

    (Sanghavi et al., 2016)
    Allele components
    Component
    Use(s)
    Regulatory region(s)
    UASp
    Encoded product / tool
    egl
    Tagged with
    EGFP
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescues

        eglR.UASp.EGFP/Scer\GAL4VP16.mat.αTub67C rescues eglGL01170

        (Sanghavi et al., 2016)
        Comments

        The mRNA (osk, grk and bcd), protein (stau, Khc, Dynein and BicD) and the oocyte nucleus localization defects as well as the disrupted microtubule network organization observed in stage 10 egg chambers of flies expressing eglGL01170 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 can be rescued by co-expression of RNAi-resistant eglR.Scer\UAS.P\T.T:Avic\GFP-EGFP.

        (Sanghavi et al., 2016)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        eglR.Scer\UAS.P\T.T:Avic\GFP-EGFP
        eglR.UASp.EGFP
        Name Synonyms
        Secondary FlyBase IDs
          References (1)