FlyBase Allele Report: Dmel\caz[KO]

General Information

Symbol

Dmel\caz^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0323134

Feature type

allele

Associated gene

Dmel\caz

Associated Insertion(s)

TI{TI}caz^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Frickenhaus et al., 2015)

Mutagen

ends-out gene targeting

(Frickenhaus et al., 2015)

Progenitor genotype

Associated Insertion(s)

TI{TI}caz^KO

Cytology

Description

caz exons 2 through 7 have been replaced by a w^+* marker flanked by inverted attP sites.

(Frickenhaus et al., 2015)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:16,288,978..16,293,530 [+]

Comment:

Region deleted via homologous recombination and replaced with a w^+* marker flanked by inverted attP sites. The extent of the deleted sequences was determined from reported primer sequences.

(Frickenhaus et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 6

CEA

(Mallik et al., 2018, Frickenhaus et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 6

is ameliorated by caz^{UAS.x10.Tag:FLAG}

(Frickenhaus et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155, caz^{UAS.x10.Tag:FLAG}

(Mallik et al., 2018)

abnormal locomotor behavior | larval stage

(Mallik et al., 2018)

lethal - all die during pupal stage

(Mallik et al., 2018)

short lived | adult stage, with Scer\GAL4^elav-C155, caz^{UAS.x10.Tag:FLAG}

(Mallik et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

caz^KO hemizygous males bearing one copy of caz^+tMa do not show any obvious adult lifespan or climbing ability (i.e. speed), as compared to controls. Adult caz^KO hemizygous males expressing caz^{UAS.x10.Tag:FLAG} under the control of Scer\GAL4^elav-C155, however, exhibit significant decreases in lifespan and climbing ability (i.e. speed), as compared to controls.

caz^KO mutant larvae show a significant decrease in larval crawling ability (i.e. speed), as compared to controls.

(Mallik et al., 2018)

caz^KO hemizygous males exhibit lethality during P-stage, with a subset of animals developing to pharate adults that fail to eclose from the pupal case, and a very small fraction of adult escapers.

(Frickenhaus et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

caz^KO has lethal - all die during pupal stage phenotype, suppressible | partially by Xrp1[+]/Xrp1^Ex-long

(Mallik et al., 2018)

caz^KO has lethal - all die during pupal stage phenotype, suppressible by Df(3R)Xrp1-Plus/+

(Mallik et al., 2018)

caz^KO has lethal - all die during pupal stage phenotype, suppressible by Xrp1^KO/Xrp1[+]

(Mallik et al., 2018)

caz^KO has abnormal locomotor behavior | larval stage phenotype, suppressible by Xrp1^KO/Xrp1[+]

(Mallik et al., 2018)

caz^KO has abnormal locomotor behavior | larval stage phenotype, suppressible by Xrp1^HMS00053/Scer\GAL4^Toll-6-D42

(Mallik et al., 2018)

caz^KO has lethal - all die during pupal stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Df(3R)Xrp1-Plus/Xrp1^{3xUAS.S.Tag:HA}/+

(Mallik et al., 2018)

caz^KO has lethal - all die during pupal stage phenotype, suppressible by Scer\GAL4^elav-C155/Xrp1^{Mut.3xUAS.S.Tag:HA}/Df(3R)Xrp1-Plus/+

(Mallik et al., 2018)

Enhancer of

Statement

Reference

caz^KO/caz^KO is an enhancer of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav-C155, Xrp1^HMS00053

(Mallik et al., 2018)

Other

Statement

Reference

Xrp1[+]/Xrp1^Ex-long, caz^KO has partially lethal - majority die phenotype

(Mallik et al., 2018)

Df(3R)Xrp1-Plus/+, caz^KO has short lived phenotype

(Mallik et al., 2018)

Scer\GAL4^elav-C155, Xrp1^HMS00053, caz^KO has short lived phenotype

(Mallik et al., 2018)

Scer\GAL4^elav-C155, Xrp1^GD9476, caz^KO has short lived phenotype

(Mallik et al., 2018)

Df(3R)Xrp1-Plus/+, Scer\GAL4^elav-C155, Xrp1^{3xUAS.S.Tag:HA}, caz^KO has partially lethal - majority die phenotype

(Mallik et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

caz^KO males rescued to adulthood by Df(3R)Xrp1-Plus heterozygosity or by the expression of either Xrp1^HMS00053 or Xrp1^GD9476 under the control of Scer\GAL4^elav-C155 are short lived and show significant decreases in adult climbing ability (i.e. speed), as compared to controls.

(Mallik et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

caz^KO is rescued by caz^+tMa

(Mallik et al., 2018)

Partially rescued by

caz^KO is partially rescued by Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG}

(Mallik et al., 2018)

caz^KO is partially rescued by Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG}

(Frickenhaus et al., 2015)

Comments

Expressing caz^{UAS.x10.Tag:FLAG} under the control of Scer\GAL4^elav-C155 fully rescues caz² hemizygous males' pupal lethality, the resulting adults exhibit an apparently normal lifespan, but exhibit a significant decrease in climbing ability (i.e. speed), as compared to controls.

(Mallik et al., 2018)

Expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 partially rescues the lethality seen in caz^KO hemizygous males.

(Frickenhaus et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

caz^KO

(Catinozzi et al., 2020, Mallik et al., 2018, Frickenhaus et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close