Expression of Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK}, either pre- or post-synaptically, under the control of Scer\GAL4^elav-C155 or Scer\GAL4^G7 respectively, does not cause any change in EJC amplitude.

Expression of Hsap\APP^{Aβ40.Scer\UAS} under the control of Scer\GAL4^ChAT.7.4 does not result in a significant decrease in lifespan or climbing ability, nor any obvious defects in neuronal morphology, as compared to controls.

2- to 3-day old adults expressing Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} under the control of Scer\GAL4^elav-C155 do not show any significant defects in a Pavlovian olfactory learning assay. The flies being to show a subtle, but statistically significant, learning defect at 6-7 days old, and the decline is most obvious in 14-15 day old flies. Learning defects are seen only in males but not in females (consistent with higher expression of Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} in the male flies). Odour avoidance (to methylcyclohexanol) and electric shock reactivity is normal in 14- to 15-day old flies expressing Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} under the control of Scer\GAL4^elav-C155. Adults expressing Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} under the control of Scer\GAL4^elav-C155 show the same climbing ability as control flies, even 3 weeks after eclosion. The lifespan of flies expressing Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} under the control of Scer\GAL4^elav-C155 is the same as that of control flies. Aged adult flies expressing Hsap\APP^{Aβ40.Scer\UAS.T:Rnor\PENK} under the control of Scer\GAL4^elav-C155 do not show neurodegeneration in the brain.