FB2025_01 , released February 20, 2025
Allele: Dmel\dcoK38R.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
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General Information
Symbol
Dmel\dcoK38R.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
Species
D. melanogaster
Name
FlyBase ID
FBal0324774
Feature type
allele
Associated gene
Dmel\dco
Associated Insertion(s)
Carried in Construct
P{UAS-dco.KR.myc.stNLS}
Key Links
Transgenic product class
dominant_negative_variant
(Venkatesan et al., 2015)
missense_variant
(Venkatesan et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Venkatesan et al., 2015)
missense_variant
(Venkatesan et al., 2015)
Mutagen
in vitro construct
(Venkatesan et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-dco.KR.myc.stNLS}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of dco that carries the amino acid replacement K38R (producing a dominant negative protein). The protein is tagged at the C-terminal end with Tag:MYC and with the Tag:NLS(SV40-largeT) nuclear localisation signal.

(Venkatesan et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dco
Tagged with
Tag:MYC
Tag:NLS(SV40-largeT)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of dcoK38R.UAS.Tag:MYC,Tag:NLS(SV40-largeT) under the control of Scer\GAL4tim.PE leads to a decrease in circadian rhythmicity and to a significant increase in circadian period, as compared to controls.

      (Venkatesan et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dcoK38R.Scer\UAS.T:Hsap\MYC,T:SV40\nls-largeT
      dcoK38R.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)