FB2025_01 , released February 20, 2025
Allele: Scer\GAL4DBD.R75G12
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General Information
Symbol
Scer\GAL4DBD.R75G12
Species
S. cerevisiae
Name
FlyBase ID
FBal0326457
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R75G12-GAL4.DBD}
Also Known As
R75G12-ZpGdbd
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wu et al., 2016)
Progenitor genotype
Carried in construct
P{R75G12-GAL4.DBD}
Cytology
Description

The R75G12 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
GMR75G12
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lobula plate-lobula columnar neuron LPLC2

      Comment: when combined with P{R19G02-p65.AD} (combination referred to as 'OL0048B')

      (Klapoetke et al., 2022, Wu et al., 2016)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Ctet\tetXTNT-LC.UAS or Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults significantly reduces takeoff rates in response to looming stimuli and almost completely abolishes short-mode takeoffs, compared to controls; in the case of inhibition using Hsap\KCNJ2IVS.10xUAS.EGFP), the giant fiber neuron has significantly reduced excitatory responses to looming and reduced depolarisation in response to slow angular velocity stimuli, compared to controls, with no effect on tonic hyperpolarisation.

      (Ache et al., 2019)

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 595 nm (amber) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults results in a large and rapid depolarization of the giant fiber cell, which does not occur in controls.

      Inhibition (using Ctet\TeTxLCTNT.UAS, but not Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults significantly reduces jump rate in response to looming stimuli, compared to controls.

      (Klapoetke et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Wu et al., 2016)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R75G12.T:Zzzz\Zip-
      Scer\GAL4DBD.R75G12
      Name Synonyms
      Secondary FlyBase IDs
        References (10)