FB2025_01 , released February 20, 2025
Allele: Dmel\PolG1UASp.RR.Tag:FLAG
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General Information
Symbol
Dmel\PolG1UASp.RR.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0327355
Feature type
allele
Associated gene
Dmel\PolG1
Associated Insertion(s)
Carried in Construct
P{UASp-PolG1.RR.FLAG}
Key Links
Transgenic product class
cDNA
(Yu et al., 2017)
wild_type
(Yu et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Yu et al., 2017)
wild_type
(Yu et al., 2017)
Mutagen
in vitro construct
(Yu et al., 2017)
Progenitor genotype
Carried in construct
P{UASp-PolG1.RR.FLAG}
Cytology
Description

UASp regulatory sequences drive expression of tam cDNA that has silent mutations in the 2074-2750 region to render it RNAi resistant to tamKK100485, tagged at the C-terminal with 3xTag:FLAG.

(Yu et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
PolG1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      PolG1UASp.RR.Tag:FLAG/Scer\GAL4VP16.bam rescues PolG1KK100485

      (Yu et al., 2017)
      Comments

      The failure to eliminate mitochondrial nucleoid and mtDNA during spermatid development characteristic for males expressing tamKK100485 under the control of Scer\GAL4bam.T:Hsim\VP16 can be fully rescued by co-expression of tamUASp.RR.Tag:FLAG.

      (Yu et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PolG1UASp.RR.Tag:FLAG
      tamScer\UAS.P\T.RR.T:Zzzz\FLAG
      tamUASp.RR.Tag:FLAG
      tamWT
      (Yu et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)