FB2025_01 , released February 20, 2025
Allele: Dmel\Nup62TRiP.cUa
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General Information
Symbol
Dmel\Nup62TRiP.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0340160
Feature type
allele
Associated gene
Dmel\Nup62
Associated Insertion(s)
Carried in Construct
P{TRiP.Nup62.U}
Key Links
Transgenic product class
RNAi_reagent
(Breuer and Ohkura, 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Breuer and Ohkura, 2015)
Mutagen
in vitro construct
(Breuer and Ohkura, 2015)
Progenitor genotype
Carried in construct
P{TRiP.Nup62.U}
Cytology
Description

UAS regulatory sequences drive expression of a Nup62 RNAi construct.

(Breuer and Ohkura, 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nup62
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Nup62TRiP.cUa under the control of Scer\GAL4unspecified leads to excessive chromatin positioning in the nuclear periphery in nurse cells and an increased nuclear diameter in oocytes, but it does not disrupt overall structural integrity of the nuclear pore complex or the active import function.

      (Breuer and Ohkura, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The karyosome disruption - aberrant attachment of the chromatin to nuclear periphery - characteristic for oocytes expressing Nup62TRiP.cUa under the control of Scer\GAL4unspecified is significantly improved by co-expression of Nup154HMS01189 and further worsened by co-expression of CG14215dsRNA.UAS, while co-expression of either Nup50HMS01054 or Nup160HMS00385 do not significantly alter the phenotype.

      The excessive chromatin positioning in the nuclear periphery in nurse cells of Nup62TRiP.cUa-expressing females is also rescued by co-expression of Nup154HMS01189.

      The increased nuclear diameter observed in oocytes expressing Nup62TRiP.cUa under the control of Scer\GAL4unspecified is not reduced by co-expression of Nup154HMS01189 (which on its own actually also increases the nuclear size).

      (Breuer and Ohkura, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Nup62TRiP.cUa
      Name Synonyms
      Secondary FlyBase IDs
        References (1)