FlyBase Allele Report: Dmel\ncd[RNAi.UAS.V22]

General Information

Symbol

Dmel\ncd^RNAi.UAS.V22

Species

D. melanogaster

Name

FlyBase ID

FBal0341665

Feature type

allele

Associated gene

Dmel\ncd

Associated Insertion(s)

Carried in Construct

P{UAS-ncd.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Radford et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Radford et al., 2017)

Mutagen

in vitro construct

(Radford et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-ncd.RNAi}

Cytology

Description

UAS regulatory sequences (from pVALIUM22) drive expression of an shRNA that targets ncd.

(Radford et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ncd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

meiotic spindle | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

Detailed Description

Statement

Reference

The expression of ncd^{dsRNA.shRNA.UAS.V22} under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to a low frequency of meiotic spindle and centromere asymmetries in late oocytes, but does not significantly affect karyosome compaction, as compared to controls.

(Radford et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, ncd^RNAi.UAS.V22 has meiotic spindle | oogenesis phenotype, non-suppressible by Klp61F^HMS00552, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

Suppressor of

Statement

Reference

ncd^RNAi.UAS.V22, Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of karyosome phenotype of Klp61F^HMS00552, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

ncd^RNAi.UAS.V22, Scer\GAL4^{VP16.mat.αTub67C} is a suppressor | partially of chromosome | oogenesis phenotype of Klp61F^HMS00552, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

NOT Suppressor of

Statement

Reference

ncd^RNAi.UAS.V22, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of meiotic spindle | oogenesis phenotype of Klp61F^HMS00552, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ncd^RNAi.UAS.V22

ncd^{dsRNA.shRNA.UAS.V22}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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