UASp regulatory sequences drive expression of the full-length, wild-type α-Cat coding sequence (amino acids 1-917), tagged at the C-terminal end with Tag:HA. The α-Cat sequence has been modified to render it resistant to RNAi by either P{UAS-α-Cat.RNAi.1} (5'-ATGTTAAAA-3' changed to 5'-ATGCTGAAG-3' at the start of the coding region) or P{TRiP.HMS00317} (5'-GCAGCATCGATATTGACTGTT-3' changed to 5'-GCCGCCTCCATCCTGACCGTG-3' in the M2 domain).
α-CatHMS00317, α-CatUASp.R.Tag:HA, Scer\GAL4VP16.mat.αTub67C has embryonic head phenotype, enhanceable by cnoTRiP.cUa, Scer\GAL4VP16.mat.αTub67C