FlyBase Allele Report: Dmel\uno[cc1]

General Information

Symbol

Dmel\uno^cc1

Species

D. melanogaster

Name

FlyBase ID

FBal0363347

Feature type

allele

Associated gene

Dmel\uno

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Weber et al., 2020)

Progenitor genotype

Cytology

Description

Intragenic, out-of-frame deletion within the coding sequence of uno. The deletion starts after codon 2.

(Weber et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:8,098,069..8,098,313 [-]

Comment:

Intragenic, out-of-frame deletion within the coding sequence of uno. The predicted protein product still has the first two aa of uno (MP) fused by an out of frame deletion to 35 extra aa (DCEESSTALLCRCQSPNPTGQEHPDEYIFFKSSGW*) before running into a stop.

(Lehner, 2021.1.5)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male, with Scer\GAL4^VP16.bam, uno^nc.UAS.EGFP

(Weber et al., 2020)

abnormal meiotic cell cycle | male (with Df(2R)Exel7094)

(Weber et al., 2020)

female fertile (with Df(2R)Exel7094)

(Weber et al., 2020)

male semi-sterile (with Df(2R)Exel7094)

(Weber et al., 2020)

viable

(Weber et al., 2020)

viable (with Df(2R)Exel7094)

(Weber et al., 2020)

Phenotype Manifest In

nucleus | male (with Df(2R)Exel7094)

(Weber et al., 2020)

spermatid (with Df(2R)Exel7094)

(Weber et al., 2020)

spermatocyte (with Df(2R)Exel7094)

(Weber et al., 2020)

testis, with Scer\GAL4^VP16.bam, uno^nc.UAS.EGFP

(Weber et al., 2020)

testis (with Df(2R)Exel7094)

(Weber et al., 2020)

Detailed Description

Statement

Reference

uno^cc1/Df(2R)Exel7094 testes exhibit bi-lobed individual chromosome territories at the late spermatocyte stages, an invariably increased number of chromosome clumps (with a reduction in their size) during pro-metaphase I, less homogenous size and DNA content of the spermatid nuclei in post-meiotic cysts, and random segregation of chromosomes during meiosis I when compared to controls.

Expression of uno^nc.UAS.EGFP under the control of Scer\GAL4^VP16.bam leads to defective bivalent separation during meiosis I in uno^cc1/uno^cc1 testes when compared to controls.

(Weber et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions