FlyBase Allele Report: Hsap\AP2M1[TA.UAS.Tag:HA]

General Information

Symbol

Hsap\AP2M1^{TA.UAS.Tag:HA}

Species

H. sapiens

Name

FlyBase ID

FBal0369263

Feature type

allele

Associated gene

Hsap\AP2M1

Associated Insertion(s)

Carried in Construct

PBac{UAS-hAP2M1.TA.HA}

Key Links

Transgenic product class

missense_variant

(Liu et al., 2021)

polypeptide_post_translational_processing_variant

(Liu et al., 2021)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Liu et al., 2021)

polypeptide_post_translational_processing_variant

(Liu et al., 2021)

Mutagen

in vitro construct

(Liu et al., 2021)

Progenitor genotype

Carried in construct

PBac{UAS-hAP2M1.TA.HA}

Cytology

Description

UASt regulatory sequences drive expression of phospho-deficient (contains the T156A mutation) Hsap\AP2M1, tagged at the N-terminal with Tag:HA.

(Liu et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\AP2M1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

decreased cell number | adult stage, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Phenotype Manifest In

dopaminergic PPL1 neuron | decreased number, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

dopaminergic PPM1 neuron | decreased number, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

dopaminergic PPM2 neuron | decreased number, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

dopaminergic PPM3 neuron | decreased number, with Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Detailed Description

Statement

Reference

Expressing Hsap\AP2M1^{TA.UAS.Tag:HA} under the control of Scer\GAL4^Ddc.PL leads to fewer PPM1/2, PPM3 and PPL1 da neurons.

(Liu et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is an enhancer of decreased cell number | adult stage phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

NOT Enhancer of

Statement

Reference

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of decreased cell number | adult stage phenotype of Hsap\LRRK2^UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\LRRK2^UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is an enhancer of dopaminergic PPM1 neuron | decreased number phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is an enhancer of dopaminergic PPM2 neuron | decreased number phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

NOT Enhancer of

Statement

Reference

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPM3 neuron | decreased number phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPL1 neuron | decreased number phenotype of Hsap\LRRK2^GS.UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPM3 neuron | decreased number phenotype of Hsap\LRRK2^UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPM1 neuron | decreased number phenotype of Hsap\LRRK2^UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Hsap\AP2M1^{TA.UAS.Tag:HA}, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPM2 neuron | decreased number phenotype of Hsap\LRRK2^UAS.cLa, Scer\GAL4^Ddc.PL

(Liu et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Hsap\AP2M1^{TA.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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