FB2025_01 , released February 20, 2025
Clone: Dmel\AT27510
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General Information
Symbol
Dmel\AT27510
Species
D. melanogaster
Name
AT27510
FlyBase ID
FBcl0018271
Feature type
cDNA_clone
Computed gene(s)
Dmel\car
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      478
      NCBI
      BF490882
      5' sequence dataSequence Downloader
      GTTTAATCAACAAAATTTATATCTGGCAACTGTTTTGATTTTAAATTGCAGGCAAGTGATAGTGTGAATGGCCCATAGAG
CAGCGGACAGTGTGACCGTGCCATAAGGCCGAAATCCGTGGGCGTTGCACCAACACCACCAACGCACTGCACAAACCGCC
CACCAAGGACACGGTAGCGACCGACCGGAAGCCGCAGAGCATACGAATCAACACTTCTGGCAAGAGGAAAGGAGCATGTT
CCCCCACTTGAAGGGCCATGGTCAGCGGGTCAACCTGCAGTTGCTGCAGGAGGCCGCCTGCCGCGAGCTGCTGCAGCAGC
TGGACCGCATTGAGGGTTCCAAGGTTATTGTGCTGGACGAGACCATGATCGGACCGCTGGACTTGGTTACCCGGCCAAAG
TTATTTGCTGATCGAGGCATACGTCTGCTGGCCCTCAAGCCGGAGCTTCATTTGCCGTGCGAGGTGGCCATTGTGGTG
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)