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General Information
Symbol
Dmel\DPiM_RH08077
Species
D. melanogaster
Name
DPiM_RH08077
FlyBase ID
FBcl0738545
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    DPiM_cDNA_bait_cDNA
    • Cell line expression plasmids encoding full-length open reading frames with C-terminal FLAG-HA tags under control of the metallothionein promoter.
    Strain
      Tissue Source
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      5,460
      NCBI
      Full Length sequence dataSequence Downloader
      GGACTGAACATATGTTCCGGGGAGAGTGGCTGCGATTTCGCGTCGGTAAAAATAGCAAATACTCGTTAATGTGCTGTGGG
      AACGCTTCCTCCCCGGCCCCAAAGTGGCCCCGAAGAAAGTGAGCAAATGTGCGCGCCGCAAGATAGTCGCCGCCGAACAA
      ACGATAGTGACGAAATGATTTAATTCAACTACCAGCACTCCCGCAAATACGATGAGTATGTCGCGCGGCGGCAACACAAC
      TCTGGACTTGCAGCCGCTCCTGGCGGAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATC
      GGTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTCGGCGTTGTTCGTCAAGAAG
      ATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGATTACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTT
      CCCCTCGTTCCTGTTCCTCAGCCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGG
      TGAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATTTCTGGGAAACATGATGTTT
      GGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGCAGCCCTACGACGCTTCTCTATCCTGATGACCATGCTGCT
      GGAGCTCAAGATCCTGGGACTGCGACCTTCGAATGCGGTTCAGGTCAGCGTATACGCAATGATCGGTGGAGCGCTGCTGG
      CCGCCTCTGATGATCTGTCCTTCAACATGAGGGGCTACATCTATGTGATGATCACTAACGCCTTGACCGCCTCGAATGGC
      GTATATGTGAAGAAAAAACTCGACACCTCGGAGATCGGAAAGTACGGCCTAATGTACTACAACTCGCTGTTTATGTTTCT
      GCCTGCCCTGGCCCTCAACTATGTTACAGGGAATCTAGATCAGGCGCTGAACTTTGAACAATGGAATGACTCAGTGTTTG
      TGGTGCAGTTCCTGCTCAGTTGCGTTATGGGTTTCATCCTATCGTACAGCACCATCCTGTGCACGCAATTCAACTCGGCG
      CTGACCACCACCATTGTGGGATGCCTGAAAAACATCTGCGTAACATATCTGGGCATGTTCATTGGAGGCGACTACGTCTT
      CTCGTGGCTCAACTGTATTGGGATCAACATCAGCGTGCTGGCTAGTCTGCTCTACACGTACGTCACTTTTCGGCGGAAGC
      GGGCTCCCGATAAGCAGGACCACTTGCCCAGCACCCGCGGCGAGAATGTCTAGCTTCATCTTAGCTCAAACTCAAGCCAA
      AGCCATACGATATGTTAAGTGTATTCCGTACTGTGTACTTCAATTGCGATCTGCGATTCGCGACAACGTTCGTATTTGAT
      TTTCCTACAACCACACAGCGCATCGTCTCTGTACATATATCTAGCTATATTTCTATAAAAATGTAATTTAGCAATTATGT
      AATTTTAATCACTTTGTACATTTGTCTGCATGCAACTGTTTCCTGCATCGAAAGTGTCATAAGAAATAGAAATACACAGG
      CTTCCACTCCTCCGCCTACGGAATGGGTGGGCCAGATAAACAATTGTGTTCCGTCTTCTGTTCCATTTTGGGTCATAATT
      GATAAGATTGCCTGCTCCGATAAGGCATACAACTCGGACGTTGCAAATTAAGCAGACATCTTGCTCTCTCTCACGTGCTT
      TTCTAATTACTTTTTTGTGTAAAATAAGTAATGTAGTGGAACTGCGTTGTGGGATTTGCCAATTACTTGTGCTTAATAGG
      TTGTTTATCTATTCTGAGCAAACACGTTGGCGCCTTGAGATTAATACAAAACAAAGTACTGCATTGCCGGACAATCCAGC
      AGCGTCATCACTCAACATCACTATGTAAAAAGCTTTGGATAGGTTGCTCGATTACTGATTACTCTATATCAAAATTTTGG
      GTTTTTATGTTGAATAAAAAAAAAACAATATTATCAGCCAAGAACGTGATTGCGAGTGGTGATAGTATTTTTTAGAATAG
      TACGCCTTCCGATATTTTGTGTTATTTTCCGTTTCATTTGGTAATTGCCTCAAAAGGCTTTTGTTTAGATTGTAGTGCTG
      AAGAATCTTTTTCTTTGTCTTAAAAGTAAATAAATTTGCGCAGCTGAAACTGTTTTGTTTTAGCGAAATAAGTATGTATA
      CTTTAAGATGGCTATCTATTTTCTTAGAATTAGATTTTACTTGGTAACTGCCTAGTTCCTTATCAAAAGATTAAGATTTA
      AATATCCCACATATATGTTGCTAAACTTATCTTTTAAATGGTGACTACTCATTTATCCAAAGAAACAACAATGACGACAT
      TGAATCGGGTATAAAGCGGCAGAATGAGGTGCTAGTGCCCCGGCAGCACTTTGTTTGGGTTATTTTGGGAGCCCGCAATA
      TATGATGGGCGGTGGTGAAGTGAGGGATTCGGAGTGGGGACAGGTAATGAGGAAGCCGAAACAGGTTTGCACAGACTTAG
      CCGTGCGGTGAGGTAAATTGTTAGTCAGCATCAGCCCCGACCGCGTACCCACATTATGGACAAATTGTTTGCTCATATTC
      GCCGCTATTGAAAGTGGGCACGTACATACGTACGTATGGCTCGAGGGACCAGCTTTAACCGAAGACAGGTAGTCAGGGTC
      ATGTTTGTGTGCGACGATGATGATGATGATAATAATAATGTAATGATGATGATGCCGACGATCGTGCTGATAAGGTCACC
      ACCGGGTGCACCTCCCCATATCTCCTCTATGAATCCACAGATCATCATGATGCAACGCTGTTTGCGTCTCCAAAAGCCGC
      TGGCCCTACGACGAGGCCTTCGCTTGGCCCAGGCTAATAGCCAAGCGGTGGCCACAGAAGCACCCGAGGCGGAACCTCTG
      GATGCCTTCGAGCGTCAGTACCTCAAGGAGCGAATCGAGATATCGCCCTTCCAGCGGCTGTTCCTGGGGGCAGGATCCTC
      CATTGCCGCGCTCTTGAATCCAAGACGTCATGACATGATCGCCTGTTTGGGTGAGACGACTGGAGAGGATGCTCTATGGA
      CAATTTTGGACACCATGCAAGCCAGCGAGGAGGGTCAGCGCATCATGGCTGATAAGCCCCGCATCCACACCAGCACCATT
      GACTTCAAGTATCTGGAAACTTTGCCACCGGATACCTTTGGAGCCGCTTACGTAAAGTTTCTGAAAGATAATCAAGTCAC
      GCCGGACAGTCGCATGGCCGTGCGGTTTTTGGAGGACCCGAAGTTGGCCTACTTGATGACGCGATATCGCGAATGCCACG
      ACCTAATCCACACGGTGCTGGACATGCCCACCAATATGCTGGGCGAGGTGGCTGTCAAGTGGGTGGAGGCTCTGAACACG
      GGCCTGCCCATGTGCTACGGTGGTGCGGTATTCGGAGCAGTTCGCCTACGTCCCAAGCAGCGGCGTGCGTACTTGAAGCA
      TTACTTGCCGTGGGCCCTGGAGAACGGCAAGCGGACCAAGCCCTTGATGCCCGTCTACTGGGAGAAGCGCTGGGAGCAGA
      ACATCCACGAGCTGCGCTCCGAATTGGGCATTACTGTGCTGAATAAAGCTTGATAAGGCTGTTTATTTTTAAGCCCATTT
      TTAAATTTTGAACTTATCGACTATTAGTATCGAGCAGTCCTATCGCGATCGTAGAATTGTGATCTGGCAACGTCGTGTAA
      CGTCGAACTGATTGTAGATGTTTCCACTTACAAAGAAAATTATTATTTGATTATAGCAATAAATATAAAAGTTGATGCAG
      TTTGCCGAAATAATTTAAAGATCGAGAAAAAAAACTTTATATTATTTTTTATATTAGAATTTGAGCACAGTGAGAACAGT
      CAATTTCCGCGAATGATCGCATCAAAACTTAACCCGATGTGGCAACTCCACTCGTTCTGTTTGCATTTGCTGATGTGTTG
      TTTTGTTTTTTTCGGTCGATCGTCTTAATGGCTAATTATCCCTAAGTTAAACACCATGACTGAGAAATTCAATGGTTTTA
      GCCACGACGAGATCCTCAAGATAACGGGCGTGAAGGAGGGCGGTGTGGGCAAAAGGCCTGCGAGTCTGGAAGCAGGTTAG
      ATCACTAAGCATGAACCCCAAAGCGTGTAAACAATTATCTGTTTATAATAAAAACCTTTGCCTATGTGTGCGTGAGCAGC
      GAAGCCGGCGCTCCGAATTCAACCCGGCATTCGGCGTATGCCCGATAAGATCTTCCGGCAAGCGGATCAGTTACGGAAGC
      AGCAGCAGCAGCAACCACAGCAACCCATACAAAAACCGGATGTGGCGAAGAAGACCAAATCGGGAACAGCGACTCCCACG
      GAAAAGCCACCGACTCCCACTCCCGCGGCGGAGGATGACGTAGCGAACGGATCGCTAAATTTGGACCGCCCACTCTCCGA
      TTCATTAATAGAGGCACTCTACCATGGAAATGCTACAGCTAGAGATAAGAAAACTCCCGCCACGTATGCAGATGCTGAAA
      CAACGTCCACCGACGACAGTTCGATTCTGAAGATCAGCGGCGGTGACAGCCAGACAACTAGCTCCACCGATGATGGCAGC
      ATCCTGCCGAGTACCCAGGACACCTCGCCGCGGGAGCGCCTCAACACAGACTCACCTTTCAAGGGCATCTCGCTGAAGGA
      CTTCGAACAGCATCGCCGCATGATCGAGGAGCAGAACAAGCAGAAGAAGCAAATGCTGTACCAGGCCATCGAGCAGCACA
      CCCAGAAGACGGCGGCGGAGTCGCGAAAGATCGAGGAGATCCGCCACGAGCTGTCCAAGCTGGAAAGTGATCTGGCCGTG
      GACGTAGCTCTGCTGAGGAAGCAGATCGACAATGCCTGCATACATTTTGCCAATGTAGAAAAACAATACGTCAAAATCGA
      GGCTCAGTTCTTGCGGGCCAAGATAGAGCTGCACAATGCATCCGAAAAGAAGGAGCTGCTCACAGAGCATCTATGCACAG
      TAATTGCCCACAACGAGGACCGTAAAGCGCAAAAGCTTACTGAATTGATGCAGAAAGTGGGTCTGTCGCCAACCGATGAT
      TGCCAACCGATTGATTTAACTCAACAAAGCCCCTAAACACGTAACACGTGTAAATTCAACTTCAAATCATCTTTAATTCG
      AATTTTCAGCGGTGTTTGCTTAACTTGACCAAAAGTATCAATTTGTTGCTCCCTGAAATATTATATTTTTTAAATTGGAA
      AATTATGTACTAATTTTCCATCGGCTATATTGTATTGGAATTGTAAACATAAAACACGCCTGAAATATGTCGAATGCGCT
      CTGATATTAGCATGTAATTAATGTTCATAGCCTAAGTGATGAAGTGCAAATTGCAAATAAAATATGTATATGTTATGTGT
      AAAGAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Description
      Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
      Sample preparation
      Protocol
      A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
      Comment
      Reported As
      Symbol Synonyms
      DPiM_cDNA_bait
      DPiM_cDNA_bait_cDNA
      References (3)
      Research paper (2)
      Rhee et al., 2014, Cell Rep. 8(6): 2031--2043
      Transcription Factor Networks in Drosophila melanogaster. [FBrf0226298]
      Guruharsha et al., 2011, Cell 147(3): 690--703
      A Protein Complex Network of Drosophila melanogaster. [FBrf0216491]
      Personal communication to FlyBase (1)
      Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project
      DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)