FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
stau, osk
FlyBase ID
FBig0000095160
Interaction Network
Interactions Browser links
stau network
osk network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0089980-2.Y2H
Reference
Breitwieser et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

fused to LexA DNA-binding domain

osk 
osk 
stau 

Stau

prey

fused to B42 activation domain

stau 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
region
unspecified role
aa 290-606aa 290-450

necessary: deletion abolishes interaction

stau 
N-terminal region
unspecified role
aa 1-568
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA/B42.

(Breitwieser et al., 1996)

The N-terminus of the long osk protein isoform inhibits the interaction with stau protein.

(Breitwieser et al., 1996)
FBrf0089980-4.PD.A
Reference
Breitwieser et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

GST-Osk292

bait

GST tag

osk 
stau 

Stau

prey

[35]S label

stau 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
region
unspecified role
aa 292-606aa 457aa 561aa 593

necessary: the osk6B10 S457F mutation abolishes interaction

necessary: the osk3 N561Y mutation reduces interaction

necessary: the osk5 R593Q mutation abolishes interaction

stau 
N-terminal region
unspecified role
aa 1-568

necessary: deletion abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down

(Breitwieser et al., 1996)

Bait expressed and purified from bacterial cells.

(Breitwieser et al., 1996)

Prey derived from in vitro translation.

(Breitwieser et al., 1996)
FBrf0223197-5.coIP.ARRAY
Reference
Laver et al., 2013
Description
physical association
Assay
Collection
Stau_RIP
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stau 

Staufen

bait

GFP tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Laver et al., 2013)

Bound RNA identified by microarray, defined as having over five-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.

(Laver et al., 2013)
FBrf0223197-88.coIP.ARRAY
Reference
Laver et al., 2013
Description
physical association
Assay
Collection
Stau_RIP
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stau 

Staufen

bait
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Laver et al., 2013)

Bound RNA identified by microarray, defined as having over two-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.

(Laver et al., 2013)
FBrf0225715-6.coIP.PCR
Reference
Gaspar et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stau 

Stau

bait

GFP tag

osk 

oskar

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gaspar et al., 2014)
FBrf0240258-3.coIP.PCR
Reference
Lu et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
stau 

Stau

bait

GFP tag

osk 

oskar

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lu et al., 2018)
FBrf0248715-3.coIP.PCR
Reference
Mohr et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
stau 

STAU

bait

3X Flag tag

osk 

osk

prey
osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR SRS domains
mutation decreasing interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transfected construct; prey produced from transfected construct.

(Mohr et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)